Drug metabolism transporters

Tukker, G., Houston, J. B., Huang, S.-M., Optimizing drug development strategies to assess drug metabolism/ transporter interaction potential — toward a consensus, Clin. Pharmacol. Ther. 2001, 70, 103-114. 

Tucker T, Houston JB, Huang S-M. Optimizing drug development strategies to assess drug metabolism/transporter interaction potential—toward a consensus. Clin Pharmacol Ther 2001 70, 103 Br J Clin Pharmacol 2001 52, 107 Eur J Pharm Sci 2001 13, 417 Pharm Res 2001 18, 1071. 

Kashuba ADM, Park JJ, Persky AM, et al. (2006) Drug metabolism, transport and the influence of hepatic disease. In Burton ME, Shaw LM, Schentag JJ, et al., eds. Applied Pharmacokinetics and Pharmacodynamics. Principles of... 

Pharmacology, the study of agents and their actions, can be divided into two branches. Pharmacodynamics is concerned with the effects of a drug on the body and, therefore, encompasses dose-response relationships as well as the molecular mechanisms of drug activity. Pharmacokinetics, on the other hand, is concerned with the effect of the body on the drug. Drug metabolism, transport, absorption, and elimination are components of pharmacokinetic analysis. 

In summary, primary cultures of hPT cells represent a unique and highly relevant experimental model for the study of drug metabolism, transport, mechanism of action, and nephrotoxicity. Although there exist the usual cautions with primary cell culture models, and cell culture models in general, once technique is mastered and appropriate conditions are used to minimize potential issues with the culture process, the primary hPT cells have numerous advantages over other models for obtaining information about drug action that is directly relevant to humans. 

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