Drained-air dried

Drained-Air Dried (1A, IB). Individual frozen books were laid on their side, not stacked, while room temperature air was gently circulated around them. Gradually the ice thawed, and the water drained from the books and evaporated. By weighing the books periodically, one could determine when all of the flood water was removed. Both samples A and B were treated by the same technique however, it was used at two different times. 

The alkalized paper was soaked in the Kymene/sorbitol solutions for one hour, hung to drain, air dried flat on plastic screen, and then oven dried at 100°C for two hours. Kymene has a pH of 4.6-4.9. To avoid having the Kymene/sorbitol solutions reacidify the paper, they were brought to pH 7 with dilute sodium hydroxide before use. 

Rhoplex AC-22N 0.5% poly- soak, drain, air dry ethylene in bath... 

Fig. I. Crosman BB shot beads plated with bright nickel and black nickel (for color coding purposes), then coated with polycarbonate by immersing in 5% solution of methylene chloride, scattering on slick paper, and allowing to dry. Various antigens or antibodies are adsorbed onto this plastic surface the beads are soaked in a % solution of bovine serum albumin in phosphate-buffered saline, drained, air dried, and stored below 0° until needed.

Prepare a mixture of 30 ml, of aniline, 8 g. of o-chloro-benzoic acid, 8 g. of anhydrous potassium carbonate and 0 4 g. of copper oxide in a 500 ml. round-bottomed flask fitted with an air-condenser, and then boil the mixture under reflux for 1 5 hours the mixture tends to foam during the earlier part of the heating owing to the evolution of carbon dioxide, and hence the large flask is used. When the heating has been completed, fit the flask with a steam-distillation head, and stcam-distil the crude product until all the excess of aniline has been removed. The residual solution now contains the potassium. V-phenylanthrani-late add ca. 2 g. of animal charcoal to this solution, boil for about 5 minutes, and filter hot. Add dilute hydrochloric acid (1 1 by volume) to the filtrate until no further precipitation occurs, and then cool in ice-water with stirring. Filter otT the. V-phcnylanthranilic acid at the pump, wash with water, drain and dry. Yield, 9-9 5 g. I he acid may be recrystallised from aqueous ethanol, or methylated spirit, with addition of charcoal if necessary, and is obtained as colourless crystals, m.p. 185-186°. 

P-Naphthyl acetate. Dissolve 5 0 g. of p-naphthol in 25 ml. of 10 per cent, sodium hydroxide solution in a 250 ml. reagent bottle, add 60 g. of crushed ice, and 5-7 g. (5 -5 ml.) of acetic anhydride. Shake vigorously for 10-15 minutes the p-naphth acetate separates as colourless crystals. Filter with suction, wash with water, drain and dry in the air. Recrystallise from light petroleum (b.p. 60-80°) or from dilute alcohol. The yield of pure product, m.p. 71°, is 6-5 g. 

Cautiously add 250 g. (136 ml.) of concentrated sulphuric acid in a thin stream and with stirring to 400 ml. of water contained in a 1 litre bolt-head or three-necked flask, and then dissolve 150 g. of sodium nitrate in the diluted acid. Cool in a bath of ice or iced water. Melt 94 g. of phenol with 20 ml. of water, and add this from a separatory funnel to the stirred mixture in the flask at such a rate that the temperature does not rise above 20°. Continue the stirring for a further 2 hours after all the phenol has been added. Pour oflF the mother liquid from the resinous mixture of nitro compounds. Melt the residue with 500 ml. of water, shake and allow the contents of the flask to settle. Pour oflF the wash liquor and repeat the washing at least two or three times to ensure the complete removal of any residual acid. Steam distil the mixture (Fig. II, 40, 1 or Fig. II, 41, 1) until no more o-nitrophenol passes over if the latter tends to solidify in the condenser, turn oflF the cooling water temporarily. Collect the distillate in cold water, filter at the pump, and drain thoroughly. Dry upon filter paper in the air. The yield of o-nitrophenol, m.p. 46° (1), is 50 g. 

The microarray is slowly removed and allowed to drain completely see Note 34). The microarray is air-dried completely and exposed to a phosphoimaging screen suitable for P-detection. 

Extraction. Transfer 5 g sieved ( 2 mm) air-dried soil to a 100-ml glass beaker, add 20 ml M ammonium ethanoate, stir and let stand overnight. Transfer the contents to a filter funnel fitted with a 125 mm Whatman No. 44 filter paper and held in a 250 ml volumetric (graduated) flask. Wash the beaker with ammonium ethanoate reagent from a wash (squeeze) bottle to remove all the sample, then add successive 25 ml volumes of reagent to leach the soil in the funnel, allowing it to drain between additions. With the collected leachate volume approaching 250 ml, remove the funnel to a rack or... 

Air-dryed frozen sections have to be rehydrated in TBS Let the fluid drain... 

Air-dryed frozen sections have to be rehydrated in TBS Block the endogenous enzyme with 20% acetic acid for 5 min Wash well with water Let the fluid drain... 

The air-dried solid is ground well in a large mortar for about three minutes with 30ml of concentrated hydrobromic acid. The residual orange solid is thoroughly drained on the filter, and washed with cold 66% alcohol, then with 95% alcohol, and with acetone. 

The crude solid ia added to 500ml of water at 70°C and atirred continuously while the temperature is raised again to 60-65°C. The purple-red solution ia then immediately filtered by auction into an ice-cooled flask, and rapidly cooled to 5°C. The complex salt ia filtered off, drained well, and air-dried. 

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