Dose-response tests

In a review. Arts and Kuper (2007) have summarized the animal test methods, which have been used to detect immune-mediated respiratory disease. The tests for respiratory sensitization include dermal as well as inhalatory or topical exposure of mice, rats, or guinea pigs for induction and challenge, and may measure various endpoints to evaluate respiratory sensitization. The review concludes that standardized and validated dose-response test methods are urgently required in order to allow identification of respiratory allergens and to make it possible to recommend safe exposure levels for consumers and workers. 

An inadequate method of treating the insects will also result in a nonlinear line. It is assumed that the applied dose will always be proportional to the dose received. If this is not the case, however, we can see that some of the insects, probably those given the higher doses, will receive proportionally less than others. This could happen in topical applications or with residual exposure methods. Therefore, caution and care are required in performing dose-response tests in resistance studies (Terriere, 1982). 

Fig. 3 Potency and selectivity of ML-260 mlAP inhibitor. The potency of the inhibitor against miAP (o), PLAP (+) and TNAP (x) was assessed by dose-response testing in 1,536-weii format in dupiicate in lx Assay Buffer, containing 100 mM DBA, pH 9.8, 0.02 mM ZnCi2, 1 mM Mg, and 1 250, 1 1,000, and 1 2,000 diluted enzymes, respectively, in a totai voiume of 4 iiL/well. The reactions were started by addition of the CDP-star substrate to the final concentrations of 200 M for the mlAP reaction, and 250 pM for the PLAP and TNAP reactions, with the luminescence intensity measured after 30 min incubation at room T...

Van Jaarsveld, P. J., Faber, M., Tanumihardjo, S. A., Nestel, P., Lombard, C. J., and Spinnler Benade, A. J. (2005). 3-carotene-rich orange-fleshed sweet potato improves the vitamin A status of primary school children assessed with the modified-relative-dose response test. Am. ]. Clin. Nutr. 81, 1080-1087. 

Andersen KE, Liden C, Hansen J, Volund A (1993) Dose-response testing with nickel sulphate using the TRUE test in nickel-sensitive individuals. Multiple nickel sulphate patch-test reactions do not cause an angry back . Br J Dermatol 129 50-56... 

The results of the dose-response tests for the four blends showed that at higher concentrations the two-component blend is equivalent to the gland rinse in eliciting the complete sequence of male behaviors, i.e., anemotaxis, touching the source, and bending their abdomens in apparent copulatory attempts. At concentrations of less than 0.02 FGE it appears that the two- and four-component blends are less effective than equivalent amounts of the gland rinse. 

The plasma concentration of vitamin A falls only when the liver reserves are nearly depleted. In deficiency there is accumulation of apo-RBP in the liver, which can only be secreted when vitamin A is available. This provides the basis for the relative dose-response test for vitamin A status — the ability of a dose of retinol to raise the plasma concentration several hours later, after chylomicrons have been cleared from the circulation. 

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