Dopamine release from synaptosomes

Grady, S., Marks, M.J., Wonnacott, S., Collins, A.C. Characterization of nicotinic receptor-mediated [3H]dopamine release from synaptosomes prepared from mouse striatum. J. Neurochem. 59 848,1992. 

Grady SR, Murphy KL, Cao J, Marks MJ, McIntosh JM, Collins AC (2002) Characterization of nicotinic agonist-induced [(3)H]dopamine release from synaptosomes prepared from four mouse brain regions. J Pharmacol Exp Ther 301 651-60 Graham B, Redman S (1994) A simulation of action potentials in synaptic boutons during presynaptic inhibition. J Neurophysiol 71 538 49... 

Bowyer, J.F., Spuler, K.P., and Weiner, N., Effects of phencyclidine, amphetamine and related compounds on dopamine release from and uptake into striatal synaptosomes, J. Pharmacol. Exp. Ther., 229, 671, 1984. 

Recently, we (Salminen et al. 2004) evaluated the effects of deleting the a2, oc4, a5, al, (32, p3, and p4 genes on both the a-CtxMIl-sensitive and resistant components of ACh-stimulated [ H]-dopamine release from striatal synaptosomes. Deletion of the oc4 and p2 subunit genes resulted in the total elimination and a5 gene deletion produced a significant decrease in the aCtxMll-resistant... 

Sharpies CG, Kaiser S, SoUakov L, Marks MJ, Collins AC, Washburn M, Wright E, Spencer JA, Gallagher T, Whiteaker P, Wonnacott S (2000) UB-165 a novel nicotinic agonist with subtype selectivity implicates the alpha4beta2 subtype in the modulation of dopamine release from rat striatal synaptosomes. J Neurosci 20 2783-2791... 

Soliakov L, Gallagher T, Wonnacott S (1995) Anatoxin-a-evoked [ H]dopamine release from rat striatal synaptosomes. Neuropharmacology 34 1535-1541 Soliakov L, Wonnacott S (1996) Voltage-sensitive Ca channels involved in nicotinic receptor-mediated [ H]dopamine release from rat striatal synaptosomes. J Neurochem 67 163-170 Sorge R, Clarke PB (2007) Slow/low intravenous infusions of nicotine in rats a better model of smoking Soc Neurosci Abst 37 273.17... 

L. Soliakov, T. Gallagher and S. Wonnacott, Anatoxin-a-evoked 3H dopamine release from rat striatal synaptosomes, Neuropharmacology, 34... 

Soliakov, L., and Wonnacott, S. 2001. Involvement of protein kinase C in the presynaptic nicotinic modulation of [H-3] -dopamine release from rat striatal synaptosomes. British J. Pharmacol. 132, 785-791. 

The binding activity of the reverse isoxazole (76) is significantly reduced relative to that of ABT-418 (Fig. 14.8). Replacement of oxygen in ABT-418 with sulfur generates isothiazole (79) with reduced binding affinity (Table 14.9). It is interesting to note that the N-unsubstituted isothiazole (77) is sixfold more active than the isoxazole counterpart (73), but still 12 times less active than ABT-418, in terms of binding activity, and also less efficacious with respect to stimulation of dopamine release from striatal synaptosomes. 

It could not be anticipated that the extension of the alpha-methyl of MDMA to an alpha-ethyl would also attenuate the effects of the compound on dopaminergic pathways in the brain. In contrast to MDMA, MBDB has no significant effect either on inhibition of uptake of dopamine into striatal synaptosomes (Steele et al. 1987) or on release of dopamine from caudate... 

We are routinely screening compounds for ability to displace 1-125 DOI from frontal cortex homogenates. As far as the CNS stimulant effects, differentiating from psychostimulants, the present model we are using is substitution in amphetamine-trained rats, in drug discrimination. We have used synaptosomes and looked at their effect on dopamine release and reuptake. But basically they are correlative models. 

Masuoka, D.T., Alcaraz, A.R, and Schott, H.F., [3H]Dopamine release by d-amphetamine from striatal synaptosomes of reserpinized rats, Biochem. Pharmacol., 31, 1969, 1982. 

Clarke PB, Reuben M. (1996). Release of [3FI]-noradrenaline from rat hippocampal synaptosomes by nicotine mediation by different nicotinic receptor subtypes from striatal [3FI]-dopamine release. BrJ Pharmacol. 117(4) 595-606. 

Rowell PP, Winkler DL Nicotinic stimulation of [ HJacetylchoUne release from mouse cerebral cortical synaptosomes. J Neurochem 43 1593-1598, 1984 Roy A, Karoum F, Pollack S Marked reduction in indexes of dopamine metabolism among patients with depression who attempt suicide. Arch Gen Psychiatry 49 447-450, 1992... 

Bowyer JF, Weiner N (1989) K+ channel and adenylate cyclase involvement in regulation of Ca2+-evoked release of [3H]dopamine from synaptosomes. J Pharmacol Exp Ther 248 514-520. 

Desce JM, Godeheu G, Galli T, Artaud F, Cheramy A, Glowinsky J (1992) L-glutamate-evoked release of dopamine from synaptosomes of the rat striatum involvement of AMPA and N-methyl-D-aspartate receptors. Neuroscience 47 333-339. 

Dayton, M. A., Geier, G. E., and Wightman, R. M., 1979, Electrochemical measurement of release of dopamine and 5-hydroxytryptamine from synaptosomes. Life Sci. 24 917-924. 

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