DNAse hypersensitivity

Hecht, A., Berkenstamm, A., Stromstedt, P.-E., Gustaksoa J -A. Sippel, A.E. (1988). A progesterone responsive element maps to the far upstream steroid dependent DNAse hypersensitive site of the chicken lysozyme chromatin. EMBO ]., 7, 2063-73. 

Changes in chromatin stracture are a hallmark of epigenetic regnlation and developmental plasticity and can be probed on a global scale using the enzyme deoxyribonuclease I (DNase I) to selectively cut open (accessible) chromatin sites (eu-chromatin) in freshly isolated intact liver nuclei (Fig. 11.8a). This technique, known as DNase hypersensitivity site (DHS) analysis, was em-... 

Fig. 11.9 Sex-diSerential binding of signal transducer and activator of transcription 5 STAT5) to liver chromatin in male and female liver. The male plasma growth hormone GH) profile aetivates a male-specific pattern of STATS binding to liver ehromatin at sites that are more accessible in male than female liver ( male-biased DHS ), as determined by DNase hypersensitivity site DHS) anal-...

DNase 1 j Under appropriate conditions, produces j single-stranded nicks in DNA. Nick translation mapping of hypersensitive sites mapping protein-DNA interactions. 

DNase I hypersensitivity assay Locus control Region (LCR)... 

Two Regulatory Regions Located Downstream of the HIV-1 Transcription Initiation Site are Associated with DNase I-Hypersensitive Sites... 

Structural studies of the chromatin organization of integrated HlV-1 have identified two DNase 1-Hypersensitive Sites (HS4 and HS7) outside of the promoter/enhancer region in U3, that were likely to play a role in controlling viral transcription by virtue of their accessibility in chromatin (Fig. la and lb) (Verdin, 1991). 

Characterization of the DNase 1-hypersensitive site HS4 associated with the 5 untranslated region of HIV-1... 

Van Lint C, (2000) Role of chromatin in HIV-1 transcriptional regulation. Adv Pharmacol 48 121-160 Verdin E (1991) DNase I-hypersensitive sites are associated with both long terminal repeats and with the intragenic enhancer of integrated human immunodeficiency vims type 1. J Virol 65 6790-6799... 

Other recent results also suggest that the interaction of the receptor-T3 complex with the chromatin alters the local chromatin structure [59], This was shown by treating the chromatin prepared from cells exposed to T3 with DNAse I three hypersensitive sites were identified in the region spanning the transcriptional initiation site from -200 to +150. The first site was in the first intron of the gene. The... 

Henkel, G., Weiss, D.L., McCoy, R, Deloughery, T., Tara, D. and Brown, M.A. (1992). A DNase I-hypersensitive site in the second intron of the murine IL-4 gene defines a mast cell-specific enhancer. J. Immunol. 149, 3239-3946. 

Kaltschmidt, C., Muller, M., Brem, G., and Renkawitz, R., DNAse I hypersensitive sites far upstream of the rat tryptophan oxygenase gene direct develop-mentally regulated transcription in livers of transgenic mice, Mech. Dev., 45, 203, 1994. 

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