DNA replication origin

T. Taira, S. M. Iguchi-Ariga, and H. Ariga, A novel DNA replication origin identified in the human heat shock protein 70 gene promoter. Mol. Cell. Biol. 14, 6386-6397 (1994). 

D. M. Gilbert, Making sense of eukaryotic DNA replication origins. Science 294, 96—100 (2001). 

M. Mechali, DNA replication origins from sequence specificity to epigenetics. Nat. Rev. Genet. 2, 640-645 (2001). 

D. M. Gilbert (2001). Making sense of eukaryotic DNA replication origins. Science 294 96-99. T. D. Gootz (1985). Determinants of bacterial resistance to beta-lactam antibiotics. Annu. Rep. Med. Chem. 20 137-144. 

In mammalian cells, at least eight DNA polymerases are present. DNA polymerase a is involved in the initiation of DNA synthesis at DNA replication origins and lagging strand synthesis (Wang, 1991). DNA polymerase 7is a mitochondrial DNA polymerase (Wang, 1991). Recently, bypass polymerases, such as DNA polymerase V, t. and Chave also been identified (Lindahl and Wood, 1999). These DNA polymerases are capable of continuing DNA synthesis even through bulky DNA lesions—such as UV-induced pyrimidine-dimers in the template strand (Lindahl and Wood, 1999). 

Wickner, S., Hoskins, J., and McKenney, K. (1991b). Monomerization of RepA dimers by heat shock proteins activates binding to DNA replication origin. Proc. Natl. Acad. Sci. U.S.A. 88, 7903-7907. 

Walter J, Newport J. Initiation of eukaryotic DNA replication origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha. Mol. Cell 2000 5 617-627. Zou L, Stillman B. Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled... 

Burhans W. C., Huberman J. A. (1994) DNA replication origins in animal cells A question of context Science 263 639. 

Mailand, N., Diffley, J. F. (2005). CDKs promote DNA replication origin licensing in human cells by protecting CDC6 from APC/ C-dependent proteolysis. Cell, 122, 915-926. 

E. coli - The DNA replication origin of E. coli is called ori. The sequence is 245 base pairs long, with four repeats of a 9-base-pair sequence that binds to an initiation protein (the dnaA gene product). To the left of these sites, depicted in Figure 24.37, are three direct repeats of a 13-base-pair sequence that is rich in A and T and is thus denatured relatively easily. The sequence also contains binding sites for several basic proteins (HU and IHF) that facilitate DNA bending, an important step in the sequence leading to initiation. Steps in the process are as follows ... 

Gilbert, D. M. Making Sense of Eukaryotic DNA Replication Origins. Science 294, 96-100 (2001). [The latest information on replication origins in eukaryotes]. 

Onn I, Milman-Shtepel N, Shlomai J. Redox potential r ulates binding of universal minicircle sequence binding protein at the kinetoplast DNA replication origin. Eukaryot Cell 2004 3(2) 277-87. 

Vieira, J., and Messing, J. (1991) New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100, 189-194. 

Fujimura, F. K., Deininger, P. L., Friedman, T., and Linney, E., 1981, Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells. Cell 23 809-814. 

---