DNA Postreplication repair

Fig. 1. Comparison of the DNA postreplication repair response in prokaryotes and eukaryotes. Shaded blocks indicate functionally conserved steps between the Escherichia coli and Saccharomyces cerevisiae pathways.

Ashley, C., Pastushok, L., McKenna, S., Ellison, M. J., and Xiao, W. (2002). Roles of mouse UBC13 in DNA postreplication repair and Lys63-linked ubiquitination. Gene 285, 183-191. 

Brown, M., Zhu, Y, Hemmingsen, S. M., and Xiao, W. (2002). Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe. DNA Repair. 1, 869-880. 

DNA adducts most likely reflects increased DNA repair such as nucleotide excision repair and postreplication repair including translesion synthesis, gap filling, and template switching during replication (27,28). 

RECOMBINATIONAL REPAIR Repair by recombination between sister DNA molecules that fills the gaps opposite unrepaired lesions left in the daughter ENA strands after replication of damaged DNA. (See also POSTREPLICATION REPAIR)... 

Despite the complexity of DNA replication in E. coli, as well as its rate (as high as 1000 base pairs per second per replication fork), this process is amazingly accurate (approximately one error per 109 to 1010 base pairs per generation). This low error rate is largely a consequence of the precise nature of the copying process itself (i.e., complementary base pairing). However, both pol III and pol I also proofread newly synthesized DNA. Most mispaired nucleotides are removed (by the 3 — 5 exonuclease activities of pol III and pol I) and then replaced. Several postreplication repair mechanisms also contribute to the low error rate in DNA replication. 

Postreplication repair refers to processes that attempt to fix mismatches, gaps, or damage to DNA that escape the repair processes that occur during replication (such as proofreading—see here). 

Postreplication repair using complementary strand from another DNA molecule... 

McDonald, J. P., Levine, A. S., and Woodgate, R. (1997). The Saccharomyces cerevisiae RAD30 gene, a homologue of Escherichia coli dinB and umuC, is DNA damage inducible and functions in a novel error-free postreplication repair mechanism. Genetics 147, 1557-1568. 

Brusky, J., Zhu, Y., and Xiao, W. (2000). UBC13, a DNA-damage-inducible gene, is a member of the error-free postreplication repair pathway in Saccharomyces cerevisiae. Curr. Genet. 37, 168-174. 

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