DNA Radiation Damage

Skov KA (1984) The contribution of hydroxyl radical to radiosensitization A study of DNA damage. Radiat Res 99 502-510... 

Waller H, Friess E, Kiefer J (1981) On the immunological detection of X-ray induced DNA damage. Radiat Environ Biophys 19 259-264... 

Berger NA Poly(ADP-ribose) in the cellular response to DNA damage. Radiat Res 1985 101 4-15. 

Radiomimetics are a class of agents that cause DNA damage of a similar quality to that resulting from exposure to ionizing radiation. 

Escherichia coli K12 TGI strain was used as a recipient for transformation. At studying of SOS-system activity the recombinant bioluminescent strain of Escherichia coli recA lux containing plasmid-borne fusions of the recA promoter-operator region to the Photorhabdus luminescens ZM 1 lux genes (GosNlIgenetika, Russia) was used. Increase of their luminescence in the presence of DNA damage factors [Rosen et al., 2000], were shown previously. Investigation of the luminescent response of this strain to UV radiation allows quantitatively estimate in a real time a SOS-system induction. 

In addition to their inherent self-sustaining properties, brain tumor stem cells may be more resistant to chemotherapy and radiation therapy than other tumor cells. Bao et al. (2006) found glioma stem cells (CD133+) were relatively radioresistant compared to CD133- tumor cells and preferentially activated the DNA damage checkpoint response. This relative resistance to standard treatment approaches of tumor stem cells compared to the majority of other cells within a tumor may underlie our current inability to cure patients with aggressive brain tumors such as glioblastoma. 

---