Dissociation constant reversed-phase liquid

The agreement between the observed and predicted k values of aromatic acids was within 10%. The correlation coefficient was 0.954 (n = 32). An error of greater than 10% for 3-hydroxy-2-naphthoic acid and 2-hydroxybenzoic acid was attributed mainly to an error in their K.A values.25 The partition coefficient, logP, and dissociation constant, pKA, of analytes can be obtained by simple calculations and by computational chemical calculations, and thus the retention time can be predicted in reversed-phase liquid chromatography. 

Bartolini M, Bertucci C, Gotti R, et al. Determination of the dissociation constants (pKa) of basic acetylcholinesterase inhibitors by reversed-phase liquid chromatography. J Chromatogr A 2002, 7 Jun 958(l-2) 59-67. 

Br —4, and 1+16 kcal mole-1)119 reflect the decreasing bond dissociation energies for H—X and C—X bonds in the series F, Cl, Br, I, and the relatively constant Z)(X—X) (Table 9.4). The highly exothermic fluorination requires no external initiators and occurs violently and uncontrollably on mixing fluorine with a hydrocarbon either in the gas or liquid phase. Chlorination must be initiated, but proceeds readily, whereas bromination frequently requires elevated temperatures. Iodination is rarely successful, and indeed is more likely to occur in the reverse direction as reduction of alkyl iodides by HI.120... 

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