Disks syringe disk

Millipore, then PE Biosystems, Framingham, MA Syringe, disk Regenerated cellulose IEX AF 19, 26-30... 

Sartobind Sartorius, Goettingen, Germany Flat sheet (syringe, disk) cylindrical Regenerated cross-linked cellulose IEX AF epoxy aldehyde IMAC 31... 

Immobilon, Intercept Q, Millipore (USA) Flat sheets, syringe, disks, PVDF, regenerated cellulose, silica, AF, lEX, RP... 

Disposable syringe disk filter holders 0.8 pm, 0.2 pm (e.g., Minisart from Sarto-rius GmbH, Gottingen, Germany). 

Remove the needle and mount a 0.8 pM followed by a 0.2-pm syringe disk filter holder. 

Figure 11.1. Format of disks used in SPE, including free disks, syringe-barrel format (syringe disk cartridge), and in 96-well microtiter plate format. (Published with permission of 3M.)...

SPE. This chapter will discuss these aspects of disk extraction with emphasis on the types of disk formats that are currently available, both free disks and disks in syringe barrels, how to use an SPE disk, examples of disk methods, automation of disks, and new technology with disks. 

The sorbents used for SPE are available in three basic formats, namely disks, prepacked cartridges, and syringe barrels, each with certain advantages. Solid media employed in SPE may be classified as nonpolar, polar and ion-exchange phases with mixed retention mechanisms (Table 3.41). The large sorbent selection ranges also... 

Syringe-barrel cartridges, disk-holders, plastic pipette-tips, well plates vacuum manifolds for semiautomatic batch processing fully automated autosamplers, xyz liquid handlers and robot-controlled work stations. 

In his work, Penefsky simply used 1 ml Plastipak tuberculin syringes fitted with porous disks cut with a cork borer (0.193 inch inside diameter) from polyethylene sheets (1.5 mm thick, 70 micrometer pore size) available from Bolab, Inc. (Deery, NH). Note The rubberized plungers of most syringes frequently contain lubricants, and these agents may bind to enzymes or they may dissolve and contaminate some enzyme preparations. 

Pack the Sepharose into a suitable chromatography column (e.g, a syringe barrel fitted with a sintered disk), and wash with 50 mL of PBS Store at 4°C in PBS containing 0 1% sodium azide. 

SPE sorbents are commercially available in three formats contained within cartridges, in columns fashioned like syringe barrels, or in disks... 

Figure 5.7. Solid-phase extraction devices come in a variety of forms, with different sorbents for different applications. Shown are packed bed with a built-in reservoir to hold the sample solution, a syringe tip packed cartridge, and a filter disk in a holder for rapid extraction from large volumes.

Electrode and Nafion film Platinum disk electrode (area = 0.28 cm2) was fabricated as previously described (27). A solution of Nafion (EW = 1100) 5% w/v in a mixture of lower aliphatic alcohols and 10% water was obtained from Aldrich and diluted with methanol to give a stock solution of 0.35% w/v. Films of Nafion-glucose oxidase were formed by syringing aliquot (20 pi) of the mixed solution (86.7 pi of Nafion stock solution and 13.3 pi of glucose oxidase solution) at the surface of the platinum electrode. The solvents were left to evaporate at room temperature for at least 30 min. The extra amount of GOD was... 

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