Discovery of Small Molecule Probes Using a Reverse Chemical Genetic Approach

1 Discovery of Small Molecule Probes Using a Reverse Chemical Genetic Approach 

The reverse chemical genetic approach begins the discovery of a small molecule modulator of a specific target protein. The small molecule is usually identified 

Small molecule microarrays were also used to discover robotnikinin (Section 1.4), a ligand that binds to the extracellular Sonic Hedgehog (Shh) protein. Approximately 10000 diversity-oriented synthesis compounds and natural products were immobilised on a single microscope slide. The small molecule microarray was challenged with the 20kDa N-terminal fragment (ShhN) of Shh some structurally related macrocycles were identified as hits and, after 

1 General Reverse Chemical Genetic Approaches for Targeting Specific Members of Families of Macromolecules 

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