Disc centrifugation, improved

An Improved Disc Centrifuge Photosedimentometer and Data System for Particle Size Distribution Analysis... 

An Improved disc centrifuge photosedimentometer (DCP) was developed for use in the determination of the particle size and size distribution of latices, pigments and other particulates. Separation is based on Stokes Law for the sedimentation of particles in a centrifugal force field and does not rely on the use of particle size calibrants or standards. The DCP Instrument provides accurate stable particle size analyses over a wide range of conditions while at the same time is rugged enough for heavy use in both a research and quality control environment. A stand-alone data collection, analysis and management system was developed both for routine quality control operation and for research use of the instrument. 

Koehler, M. E., Zander, R. A., Gill, T.T., "An Improved Disc Centrifuge Photosedimentometer and Data System", This Volume. 

COLL AND OPPENHEIMER Improved Techniques in Disc Centrifugation... 

Murray considered possible improvements in the separation efficiency of disc centrifuges by reversing the liquid flow through the disc stack but found no advantage in using this method in practice. 

Alternative means for liquid discharge have been developed, largely from other centrifuge types, such as centripetal and skimmer pumps. Decanters have been fitted with discs to improve clarification performance, and sanitary performance has been improved by the development of clean-in-place (CIP) methods. 

The RE process proceeds in three major types of equipment mixer-settler systems, column extractors, and centrifugal extractors. Countercurrent column extractors can be further subdivided into nonagitated nonproprietary columns and agitated proprietary extractors. Agitating the liquid-liquid system breaks up droplets and increases the interfacial area to improve the mass transfer and column efficiency. Various forms of energy input are used, e.g., rotation of propellers, impellers, and discs pulsation, vibration, and ultrasonic devices and centrifugal devices. 

Proteoglycans.— By using a vertical tube rotor for isopycnic centrifugation, the time taken for the isolation of proteoglycans from various tissues can be shortened and the resolution of proteoglycans is improved." Invertebral disc proteoglycans... 

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