Dioleoyl phosphoethanolamine

Fig. 19 Left) Schematic representation of the proposed mechanism for topological changes in dioleoyl phosphoethanolamine (DOPE) based liposomal membranes upon ultrasound irradiation. Right) (a) Giant DOPE-based unilamellar vesicle, before sonication, which shows an inhomogeneous membrane DOPE-rich domains of negative curvature are marked in red, embedded in zones rich in dioleoyl phosphocholine (DOPC) of zero mean curvature, (b) Illustration of shape changes upon ultrasound stimuli. Reproduced with permission from [99]. Copyright 2014 The Royal Society of Chemistry...

I,2-dioleoyl-rw-glycero-3-phosphoethanolamine, DOPE l,2-dipalmitoyl-rw-glycero-3-phosphothioethanolamine (Sodium Salt), DPPE-SH I,2-dimytistoyTOT-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (ammonium salt), Rh-PE (all from Avanti Polar Lipids). 

Dissolve in chloroform 196 mg of egg phosphatidylcholine, 45 mg of cholesterol, 5 mg of l,2-distearoyl-sn-glycero-3-phosphoethanolamine-M-[methoxy(polyethylen-eglycol)-2000] and 7. 5 mg of l,2-dioleoyl-3-trimethyl-ammonium-propane. 

DOPE l,2-Dioleoyl-sn-glycero-3-phosphoethanolamine DPPC l,2-Dipalmitoyl-SM-glycero-3-phosphocholine DPPS l,2-Dipalmitoyl-s -glycero-3-phosphoserine LB Langmuir-Blodgett MC Monte Carlo... 

Liposomes. Liposomes are vesicles that consist of an aqueous compartment enclosed in a phospholipids bilayer. As they lack proteinaceous components, liposomes are not immunogenic. A broad variety of cationic liposomes containing eventually modified lipids have been used to deliver pDNA and mRNA for therapeutic utilizations. These formulations are usually based on a cationic lipid such as l,2-dioleol-3-trimethylammonium propane (DOTAP) and a fusogenic lipid such as l,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) [22,23]. Despite their ease of production and efficacy in vitro for transfecting cultured cells, cationic liposomes face toxicity issues in vivo [24,25]. Meanwhile, their heterogeneous size... 

Figure 14 Structures of zwitterionic phospholipids commonly used in the formation of liposomes and lipid bilayers. Shown are l-palmitoyl-2-oleoyl-in-glycero-3-phosphocholine (POPC), l,2-ipalmitoyl-in-glycero-3-phosphocholine (DPPC), and 1,2-dioleoyl-iw-glycero-3-phosphoethanolamine (DOPE). As is the case with POPC and DPPC, the acyl chains attached to the polar phosphatidylcholine head group may correspond to various fatty acids, which can be interchanged. DOPE, on the other hand, is comprised of the same acyl chains as DPPC and POPC but differs in the headgroup.

PEC-PL, 1,2-dioleoyl-s r -glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-1000. ... 

Critical aggregation concentration Concanavalin A iV,A -dicyclohexylcarbodiimide l,2-Dioleoyl-j M-glycero-3-phosphoethanolamine... 

An additional method to directly conjugate antibodies to the PEG terminus of liposomes without prior derivatization has been described by Torchilin et al. [128]. The amphiphilic derivate p-nitrophenylcarbonyl-PEG-l,2-dioleoyl- n-glycero-3-phosphoethanolamine (pNP-PEG-DOPE), which was obtained in a single step from DOPE and bis(p-nitrophenylcarbonyl)-PEG, forms stable and non-toxic carbamate bonds with ligands containing primary amines (Fig. Ik). However, one drawback is the utilization of the bis-functionalized bis(p-nitrophenylcarbonyl)-PEG, which may result in dimerization, although this side product is easily... 

Vesicles were swollen from 1,2-dioleoyl-s/i-glycero-3-phosphocholine (Avanti Polar Lipids, USA) and V- (6-(biotinoyl)amino)hexanoyl)-l,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE, Molecular Probes, The Netherlands) at a ratio of 99 1. Tubes were grown out of lipid globules in shear flow and cut to the desired length with a pipette. Streptavidin-coated beads (Dynal, Germany) were used... 

FIGURE 6.24 Examples of SAXS data collected in two different lipid lamellar phases, (a) The gel phase Lp. on a sample of pure DPPC (l,2-dlpalmltoyl-sn-glycero-3-phosphocholine) and (b) in the liquid crystalline lipid phase on a sample of pure DOPE (l,2-dioleoyl-sn-glycero-3-phosphoethanolamine. Both sets of data were collected using a synchrotron x-ray source at Brookhaven National Laboratory. 

Melittin is an amphipathic peptide which has received much attention as a model peptide for peptide-membrane interactions. It is however not suited as a transfection agent due to its cytolytic and toxicologic effects. Retro-inverso-melittin, when covalently linked to the lipid l,2-dioleoyl-s -gZycero-3-phosphoethanolamine (riDOM), eliminates these shortcomings. RiDOM forms cationic nanoparticles with a diameter of 13 nm which are well soluble in water and bind with high affinity to DNA and lipid membranes. RiDOM-induced membrane leakiness is however much reduced compared to that of authentic melittin. The P NMR spectrum of the nanoparticle is however transformed into a typical bilayer spectrum. ... 

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