Gibberellic acid differentiation

DeMaggio AE (1966) Phloem differentiation induced stimulation by gibberellic acid. Science 152 370-372... 

Sorokin HP, Mathur SN, Thimann KV (1962) The effects of auxins and kinetin on xylem differentiation in the pea epicotyl. Am J Bot 49 444-453 Stant MY (1961) The effect of gibberellic acid on fibre-cell length. Ann Bot 25 453-462 Stant MY (1963) The effect of gibberellic acid on cell width and the cell wall of some phloem fibers. Ann Bot 27 185-196... 

Wangermann E (1967) The effect of the leaf on differentiation of primary xylem in the internode of Coleus blumei Benth. New Phytol 66 747-754 Wangermann E (1970) Autoradiographic localization of soluble and insoluble from [ " C]indolylacetic acid supplied to isolated Coleus internodes. New Phytol 69 919-927 Wareing PF (1958) Interaction between indole-acetic acid and gibberellic acid in cambial activity. Nature 181 1744-1745... 

Techniques of Purification. The purification techniques have included column and batch adsorption, extraction, dialysis, and column and paper chromatography. Liberal use was made of the fact that the gibberellate anion is not soluble in ethyl acetate, whereas the free acid is soluble, and that charcoal will adsorb GA3 from aqueous solutions but release it with acetone (26). As a rough rule, preliminary concentration by a factor of about 105 was necessary before significant use of paper chromatography could be made. For kudzu vine and pinto bean, a known amount of GA3 was added to an aliquot of the plant extract and taken through the same procedure as the initial extract. These controls are subsequently referred to as "spiked extracts, to differentiate them from the initial or "natural extract. 

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