Differential spectroscopy blood

The sorption efficiency of MC was determined as the ratio of the quantity of the adsorbed substance to its initial amount (w / w), expressed in % for a certain ratio (w / w) of adsorbent to substance. Optimal ratios of adsorbent to substance equal 15-20 for barbiturates, 20 - 25 for cyanocobalamin and bilirubin, and 40 - 50 for hemoglobin. The initial concentration of absorbed substances was 100 - 200 pg/ml. The substances were incubated for lmin with MC either in physiological solution or in donor plasma and donor blood at room temperature (pH 7.4). The concentration of substances in the solutions was measured by differential visual and UV-spectroscopy. Concentrations of substances in blood and plasma and adsorption of total plasma proteins was determined by thin-layer chromatography with a fluorescent label. 

There are a few sophisticated techniques utilizing biochemical analyses that can potentially determine particle reactivity under in vivo conditions. Some of these techniques include measuring hemolytic potential, which demonstrates the differential surface activity particles from non-reactive particles when incubated with red blood cells. Other techniques for measuring nanomaterial surface activity include electron spin resonance, electron microscopy, and mass spectroscopy. 

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