Dichlorobis isopropylamine platinum II

The differences revealed in the gel electrophoretic pattern of the nucleosome core particle after binding cis- and trans-DPP (10) could possibly provide a simple in vitro screen for pTatinum antitumor drug activity. Preliminary studies (12) have shown that nucleosome cores incubated with either dichloroethylenediamine-platinum(II) or cis-dichlorobis(isopropylamine)-platinum(II), two known antitumor drugs, exhibit gel electrophoretic patterns very similar to those of nucleosome cores incubated with cis-DDP. Incubation with [(terpy)PtCl]Cl, an inactive compound, gave very different gel patterns. Further work is in progress to evaluate the utility of this assay. 

In summary, the studies of the binding of cis-and trans-DPP with the nucleosome core particle have 

ACS Symposium Series American Chemical Society Washington, DC, 1980. 

The relevance of this discovery to the cytotoxicity and greater antitumor drug action of the cis isomer is not obvious. Perhaps trans-DDP, the more reactive crosslinking reagent, is scavenged vivo before it can reach the nuclear DNA. 

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