DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE DAPI

CA Index Name l//-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)-phenyl]-, hydrochloride (1 2) Other Names l//-Indole-6-carboximidamide, 2-[4-(aminoiminomethyl)-phenyl]-, dihydrochloride Indole-6-carboxamidine, 2-(p-amidinophenyl)-, 

Solubility Soluble in water, V,V-dimethylformamide, dimethyl sulfoxide, ethanol, methanol 

Biological/Medical Applications Detecting nucleic acids, cells, neuron-specific nuclear 

Sabnis, R. W. Handbook of Biological Dyes and Stains, John Wiley Sons Inc. Hoboken, 2010 pp 127-128. 

2-phenylbenzofuran, 2-phenylindene, and 2-phenylindole. Justus Liebigs Ann. Chem. 1971, 749, 68-89. 

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Cell organelle specific fluorescent markers, e.g., MitoTracker or ER-Tracker Red or 4, 6-Diamidino-2-phenylindole dihydrochloride (DAPI) optional). See Note 6. 

Inc. 1 2000) for 1 h at room temperature. The cells were washed with PBS and counterstained with DAPI (4, 6-diamidino-2-phenylindole dihydrochloride) to visualize the nuclear morphology. The immunoreactive images were acquired by a confocal laser scanning microscopy (LSM5 PASCAL, Carl Zeiss, lend, Germany). The dendritic morphology was analyzed with the aid of Neurocyte Image Analyzer Software (Kurabo, Osaka, Japan). 

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