Dialdehyde cotton gauze

Both carboxymethylated cellulose (CMC) and oxidized cellulose (oxycellulose) have been utilized in wound management either as a constituent of hydrocolloid wound dressings (14) which produce high absorbency or as a preferred hemostatic agent in surgery (15), respectively. We have investigated both carboxymethylated and dialdehyde cotton gauze in order to assess their effect on elastase sequestration. 

Weigh accurately a 200-milligram sample of dialdehyde cotton gauze and place it in a 125 mL Erlenmeyer flask. To the flask is add 10 mL of standardized 0.1 N sodium hydroxide and the flask is swirled and placed in a 70 C water bath for 60 minutes. The flask is cooled under running tap water. The residual sodium hydroxide is then titrated with standardized 0.1 N hydrochloric acid to the phenolphthalein endpoint. The percent dicarbonyl units is calculated with the following equation ... 

Figure 6 Percent levels of dicarbonyls in dialdehyde cotton gauze (DAG I and II) and carboxylates (CMC III and TV) on carboxymethylated cellulose as determined by titration on modified cotton fibers. Data are mean SE of triplicate determinations.

The gauze finishes employed in this study were prepared to assess the effect of both sterilization and variation of the sodium periodate finishing conditions on the activity of dialdehyde cotton gauze in reducing elastase activity. The reaction for... 

Figure 7 Assessment of reduction in human neutrophil elastase activity from several oxidized cotton gauze samples. Untreated cotton gauze was employed as a control Treatment 7 Treatment 2 Treatment 3. Elastase activities are compared in terms of initial velocities for solutions taken from dialdehyde cotton gauze samples. Data are mean + SE of triplicate determinations.

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