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DHPLC denaturated high performance

SSCP, single-strand conformation polymorphism PNA-LNA, peptide nucleic acid-locked nucleic acid MALDI-TOF MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ARMS, amplified refractory mutation system dHPLC, denaturing high performance liquid chromatography. [Pg.410]

One specialty in dehybridization detection is that of dHPLC (denaturing high-performance liquid chromatography). Hybridization products differ in melting temperature and mobility on a specific support if a mismatch is introduced by a SNP. In the case of a SNP, a heteroduplex is formed in the analyzed sequence, whereas in the case of identical sequences (no SNP present), a perfect homoduplex is formed. These events can be discriminated by dHPLC at a low to medium throughput for SNP discovery or SNP detection. [Pg.101]

DHPLC Denaturing High-Performance Liquid IRMA Immuno-Radiometric Assay... [Pg.2520]

Liu W, Smith DI, Rechtzigel KJ, Thibodeau SN, James CD (1998) Denaturing high performance liquid chromatography (DHPLC) used in the detection of germline and somatic mutations. Nucleic Acids Res 26 1396-1400... [Pg.547]

FIGURE 16.1 Steps of denaturing high performance liquid chromatography (DHPLC). [Pg.190]

Frueh, F. W. and Noyer-Weidner, M. (2003) The use of denaturing high performance liquid chromatography (DHPLC) for the analysis of genetic variations impact for diagnostics and pharmacogenetics. Clin. Chem. Lab. Med. 41,452-461. [Pg.95]

Hutzler, M., Geiger, E., Jacob, F. (2010). Use of PCR-DHPLC (Polymerase chain reaction-denaturing high performance liquid chromatography) for the rapid differentiation of industrial Saccharomyces pastorianus and Saccharomyces cerevisiae strains. Journal of the Institute of Brewing, 116, 464-474. [Pg.100]

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Denaturing DHPLC)

Denaturing high performance

Denaturing high performance dHPLC)

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