Determination with appropriate biological activities

Determination of Plants with Appropriate Biological Activities. The... 

Because the shape of a molecule determines whether it will be recognized by a receptor and, therefore, whether it will exhibit biological activity, compounds with similar biological activity often have similar structures. Because computers can draw molecular models of compounds on a video display and move them around to assume different conformations, computer molecular modeling allows more rational dmg design. There are computer programs that allow chemists to scan existing collections of thousands of compounds to find those with appropriate structural and conformational properties. 

A bioassay is an analytical procedure that uses a responsive biological system (biological function) to measure the biological potency of a product (Mire-Sluis et al., 1996). The most appropriate method to determine it is by comparing the biological activity of a sample with a well-characterized reference standard. 

Depending on the composition of the various membranes, it might be appropriate to use IAM columns with different phospholipids. As these columns are not commercially available, phosphatidylcholine (PC)- or phosphatidylserine (PS)-covered infusorial earth as the stationary phase and phosphate buffer as the mobile phase have been used to determine the retention time for a series of 2,3,6-triaminopyridines with anticonvulsant activity. Again, log JCPS was superior to log K oct in describing the observed variance in biological activity [7]. 

These include determination of physicochemical properties, biological activity, immunochemical properties, and purity. The product should be compared with an appropriate reference material or its natural counterpart. 

Two assays were developed that measure the potency of the FGF-4 transgene carried by Ads FGF-4. In the first case, a one-step growth-promotion assay is conducted on normal, human retinal pigment epithelial cells (ARPE-19). The assay measures metabolic activity (Alamar blue dye metabolism) following infection of ARPE-19 cells with a serial dilution of the virus. The increase in metabohc achvity was measured in relation to a mock-infected control. This increase correlates with FGF-4 produchon determined by an FGF-4 ELISA, increased de-novo DNA synthesis measured by BrdU incorporation, and an increase in cell number. This procedure is therefore an appropriate in-vitro efficacy measure, indicating that the FGF-4 transgene product is biologically active. 

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