Determination of Bioconcentration Factors

For performing the bio concentration flow-through fish test see [22]. An apparatus for continuously saturating water with hydrophobic organic chemicals was described by Veith and Comstock [49]. However, an exposure system with generator column [26,27] is recommended for very hydrophobic chemicals [28, 

During the uptake phase the concentration of the chemical in the water must be analyzed at appropriate times. For more information on the performance of the Kinetic approach see references [21,22,26,28]. For very lipophilic chemicals it is important to measure the depuration for a relatively long time (some months corrected for growth of course). Otherwise, a great elimination rate constant is calculated, and thus a small BCF value is obtained. 

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