Detector sampling rate

An example of a fast, well-executed separation is shown in Figure 8. A 2-mm i.d. 3 cm 3.5- J,m column was used in a rapid, 1-min gradient. In order to maximize the peak capacity, a high flow rate was used 2mL/min. To reduce the column backpressure, the separation was carried out at 60°C. A fast detector sampling rate was employed to keep up with the high speed of the separation. The injection was coordinated with the arrival of the gradient at the top of the column in order to eliminate the... 

The detectors used with UPLC systems have to be able to handle very fast scanning methods because peak half-height widths of around 1 s are typically obtained with columns packed with 1.7-p.m particles. In order to accurately and reproducibly integrate an analyte peak, the detector sampling rate must be high enough to capture enough data points across the peak. Conceptually, the sensitivity increase for UPLC... 

The ability to quickly ramp column temperature is an excellent way to increase analysis speed, given appropriate carrier gas flow rates and fast detector sampling rates. Along with column dimensions and operation above... 

Other factors and instrumait parts should also be taken into account to take full advantage of UPLC separations. A completely new system design with advanced technology in the solvent and sample manager, packing method, introduction method, injection valves, detector sampling rate, data system, and service diagnostics is required [50]. 

Column Column Length Column Diameter Column Packing Mobile Phase Flow Rate Detector Sample Volume... 

Mobile Phase Flow Rate Detector Sample Volume... 

Column Mobile phase Flow rate Detector Sample ... 

Another data acquisition consideration is data file size. A high speed LC/MS data file can easily reach dimensions of 20 MB/min if maximal information is required and the detectors are set to broadest scan ranges and highest sampling rates without data reduction. LC/MS systems capable... 

Sampling Detector Changes rate Changes noise level in... 

This work was done with a Waters Model 244 liquid chromatograph having two Du Pont Blmodal IIS columns (29,000 plates/meter) and a Linear dual-pen recorder. Also used was a Waters Model 440 UV absorbance detector. Samples were run at 0.1% (w/v) using an Injection volume of 25-pL and a flow rate of 1 mL/mln. The system was calibrated with polystyrene standards from Pressure Chemical Co. according to the universal callbaratlon procedure. Data collection and computation were done with an Intel 80/30 microprocessor. 

Once a source of molecules exists, that source becomes the beginning of a pathway that leads, hopefully, to the sampling portion of a sensor system. That pathway may be tortuous it may be broad. There are a number of effects that alter the rate at which molecules become available to a detector. That rate, expressed as a concentration level per unit time within the medium where the detection... 

---