Depancreatized rats

Poly(ADP-Ribose) Synthetase Inhibitors Induce Islet B-Cell Regeneration in Partially Depancreatized Rats... 

Fig. 1. Administration of poly(ADP-ribose) synthetase inhibitors to 90% depancreatized rats...

Fig. 2. Urinary glucose excretion in partially depancreatized rats with or without poly(ADP-ribose) synthetase inhibitor injection. 1, 2, and 3 months after the 90% pancreatectomy, the urine of each rat was collected for 24 h. Urinary glucose levels were measured by the glucose oxidase method. O Control rats nicotinamide-injected rats 3-aminobenzamide-injected rats. Statistical significance of differences between rats treated with and without poly(ADP-ribose) synthetase inhibitors was analyzed using Student s t test. Each point is the mean for five different rats vertical bars show SD when larger than the symbol indicating the mean value., and = p<0.10, p<0.05, and p<0.025 vs control rats. The time after the partial pancreatectomy is shown on the abscissa...

Fig. 3. Pancreatic islets in pancreata stained with hematoxylin and eosin. A Remaining pancreas from control 90% depancreatized rat. B Remaining pancreas from nicotinamide-treated rat. Magnification X 825...

Yonemura Y, Takashima T, Miwa K, Miyazaki I, Yamamoto H, Okamoto H (1984) Amelioration of diabetes mellitus in partially depancreatized rats by poly(ADP-ribose) synthetase inhibitors. Diabetes 33 401-404... 

The striking effect of lecithin upon the liver of a depancreatized dog (maintained with insulin) was found to be due to its content of choline, which reduces the fat content of the liver of depancreatized rats as well as of rats fed a diet rich in fat. The fact that the rate of formation of new phosphatide molecules in the liver is accelerated by the administration of choline, was established by Perlman and Chaikoff (139). Rats were fed for three days on a diet high in fat and low in protein on the fourth day, half of each group were given 3 mg. of labeled phosphate and 30 mg. of choline chloride, simultaneously. The remaining half was pven the labeled phosphate only. All animals were killed 4 hours after the administration of P. While the phosphatides in the livers of the controls were found to contain 2.23% of the P administered, the liver phosphatides of the choline-treated rats contained 2.92% (see also 137). Increased formation of labeled phosphatides was found to appear approximately 1 hour after choline ingestion, and its effect had disappeared about 10 to 12 hours later. 

Male Wistar rats weighing 180-200 g were 90% depancreatized according to the technique described by Foglia [13] and maintained on standard rat chow. As shown in... 

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