Deoxyribonuclease applications

Deoxyribonuclease (DNAase), an enzyme that degrades deoxyribonucleic acid, has been used in patients with chronic bronchitis, and found to produce favorable responses presumably by degrading the DNA, contributed by cell nuclei, to inflammatory mucus (213). Lysozyme [9001-63-2] hydrolyzes the mucopeptides of bacterial cell walls. Accordingly, it has been used as an antibacterial agent, usually in combination with standard antibiotics. Topical applications are also useful in the debridement of serious bums, cellulitis, and dermal ulceration. 

N. B. Kmrnick. The determination of deoxyribonuclease activity by methyl green application to serum. Arch. Blochem. 29 41-53 (1950). 

Hsu CC, Wu SS, Walsh AJ. The preparation of recombinant human deoxyribonuclease powder comparative studies of spray drying versus lyofrftilization and application of microwave drying. In Strumillo C, Pakowski 2, cds. Drying 96. Vol B. Krakow, Poland Lodz Technical University, Dep. of Process and Environmental Engineering, 1996 1229-36. 

The stability of ODN against nuclease is an important issue for in vivo application. Both the acetal-PEG-ODN conjugate and its PIC micelle with L-PEI were incubated with deoxyribonuclease (DNase I) for appropriate time intervals (5, 30, 60, and 120 min), and the digestion of ODN was quantified by means of an SEC analysis. So that an exact evaluation of the stability against DNase I attack of ODN in PIC micelles could be obtained, excess dextran sulfate (counter polyanion) was added to the PIC micelles solution. This step... 

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