Deoxyribonuclease activity

A major advantage of DNA as a carrier is that no chemical synthesis or manipulations are needed to obtain DNA-drug complex. The efficacy of the DNA-drug complex depends on stability in the bloodstream, endocytic behavior of normal and tumor cells, presence of extra-cellular deoxyribonuclease activity in the tumor tissues, and capillary barriers that separate normal and tumor cells from the bloodstream. 

Enzyme activity has been sought in seawater Strickland and Solorzano [288] looked for photomono esterase activity, while Maeda and Taga [289] used a flu-orometric method for assaying deoxyribonuclease activity in both seawater and sediment samples. 

N. B. Kmrnick. The determination of deoxyribonuclease activity by methyl green application to serum. Arch. Blochem. 29 41-53 (1950). 

Note Lysis buffer (without proteinase K) and the TE buffer should be autoclaved to remove contaminating deoxyribonuclease activity. 

Wll. Weinstock, I. M., and Lukacs, M., Enzyme studies in muscular dystrophy. VI. Cathepsin and acid deoxyribonuclease activities during the progression of hereditary muscular dystrophy in the chicken. Enzymol. Biol. Clin. 6, 103-112 (1965). 

D3. Daoust, R., and Amano, H., Ribonuclease and deoxyribonuclease activities in experimental and human tumors on the histochemical substrate film method. Cancer Res. 28, 131-134 (1963). 

Steigleder, G. K., and Fissher, I., Localization of ribonuclease and deoxyribonuclease activity in normal skin, skin altered by inflammation and in skin tumors. Arch. Klin. Expil. Dermatol. 217, 653-562 (1963). 

C2 Caldarera, C. M. and Marchetti, M. Liver ribonuclease and deoxyribonuclease activity of vitamin B 12-deficient chicks effects of orotic acid and methionine. Nature (London), 195, 703-704 (1962)... 

Among the contaminants of DNA preparations obtained from mammalian tissue, protein and RNA are the most conspicuous. Proteins can be separated by denaturation with chloroform and amyl alcohol, a procedure that may be followed by repeated washings with sodium dodecyl sulfate. RNA can be eliminated by repeated digestion of the preparation with ribonu-clease. (Even crystallized RNase has deoxyribonuclease activity therefore, the RNase preparation must be boiled for 3 minutes before using it for treating the DNA preparation.)... 

Pogo, B. G. T., and Dales, S., 1969, Two deoxyribonuclease activities within purified vaccinia, Proc. Natl. Acad. Sci. USA 63 820. 

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