Denaturing gradient gel electrophoresis DGGE

G. Muyzer and K. Smalla, Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Van Leeuwenhoek 73 127 (1998). 

Denaturing gradient gel electrophoresis (DGGE), real-time PCR, and FISH... 

B2. Borresen, A. L., Hovig, E., etal, Detection of base mutations in genomic DNA using denaturing gradient gel electrophoresis (DGGE) followed by transfer and hybridization with gene-specific probes. Mutat. Res. 202(1), 77-83 (1988). 

The fundamental need to characterise and quantify bacterial strains in natural biofilms in order to understand their potential role as producers of settlement signals highlights the need for molecular techniques which can be used to identify and quantify bacteria in situ. For example, low abundance of putatively deterrent bacterium on surfaces in situ may preclude the production of sufficient quantities of deterrent metabolites. Appropriate techniques for such characterization include denaturing gradient gel electrophoresis (DGGE)107 and fluorescence in situ hybridization (FISH)108 and would in principle allow for the detection and quantification of all species in the biofilm. 

Figure 11.10 Denaturing gradient gel electrophoresis (DGGE) showing fingerprint patterns for thirteen different soils in Lanes 3-10 and 13-17. Lanes 1, 2, 11, 12, and 18 are marker lanes. Dark bands show presence of different bacterial genotypes.

Lerner, A., Shor, Y., Vinokurov, A., Okon, Y., and Jurkevitch, E. (2006). Can denaturing gradient gel electrophoresis (DGGE) analysis of amplified 16s rDNA of soil bacterial populations be used in forensic investigations Soil Biol. Bio-chem. 38, 1188-1192. 

Nicolaisen, M. H., and Ramsing, N. B. (2002). Denaturing gradient gel electrophoresis (DGGE) approaches to study the diversity of ammonia-oxidizing bacteria. J. Microbiol. Meth. 50, 189-203. 

Dolinsky LCB. Denaturing Gradient Gel Electrophoresis (DGGE) for mutation detection in Duchenne muscular dystrophy (DMD). Methods Molec Biol 2003 217 165-75. 

One molecular method useful for the evaluation of microbial diversity is the apphcation of the Temporal Temperature Gradient gel Electrophoresis (TTGE) or the Denaturing Gradient Gel Electrophoresis (DGGE) to a PCR product (Myers et al. 1987). 

Siciliano SD et al., Assessment of 2,4,6-trinitrotoluene toxicity in field soils by pollution induced community tolerance (PICT), denaturing gradient gel electrophoresis (DGGE) and seed germination assay, Environ. Toxicol. Chem., 19, 2154, 1999. 

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