Demonstration, genomic annotation

Insight into the structural and functional determinants for enzyme specificity allowed us to access functional assignment of p-decarboxylating dehydrogenase sequences. Of the cDNA or genomic sequences that have been published or deposited in databases, at least 20 are incorrectly annotated. Case studies are presented here to demonstrate that correct annotation can be made with certainty. 

Each of the tools and databases discussed previously is grounded in a significant amount of both technical and theoretical detail. To illustrate the utility of these tools, practical data-analysis examples are provided that outline how a microarray experiment can be designed and analyzed. In addition, the annotation of an uncharacterized EST is defined and mapped to the genome. These examples also demonstrate how to assign annotation to microarray analysis, when the identify of a EST represented on an array may be unknown. 

Once there is a list of predicted RNAs, the user may want to add additional annotations to the predictions. Additional fields can be added to the tabulator separated data file. Here, we demonstrate this by comparing our prediction with the known ncRNA annotation from the Saccharomyces genome database. The program rnazAnnotate.pl checks each predicted locus for overlap with an annotation file in BED format ... 

The CYP71A subfamily contains 28 members including 17 annotations from the A thaliana genome. The first member of this subfamily was derived from avocado. No specific enzymatic activity has been demonstrated for the members of the CYP71A subfamily. CYP71A10 from Glycine... 

Human accessory chromosome (HAC) vectors, introduced in 1997 by Harrington et al. [225] demonstrated clones carrying up to 10 000 kb of DNA. These types of stable vectors are of more importance as gene transfer vectors to human cells to help annotate the human genome without disruption of the extant chromosomes and without use of potentially dangerous fragments of viral vectors. 

---