3-Dehydroshikimate dehydratase

Dehydroshikimate dehydratase, the enzyme which catalyses the conversion of 3-dehydroshikimate (57) to protocatechuate (58) in Neurospora crassa has been studied by several groups and some... 

At the beginning of 2014, Evolva and IFF announced entering the preproduction phase of natural vanillin by a new de novo process starting from glucose [199] in Schizosaccharomyces pombe, andS. cerevisiae. The engineered pathways involve the incorporation of 3-dehydroshikimate dehydratase from the dung mold Podospora pauciseta, an aromatic carboxylic acid reductase (ACAR) from... 

The reversible conversion of dehydroquinate to dehydroshikimate is catalyzed by 3-dehydroquinate dehydratase [Fig. 2 (3)]. The occurrence of this enzyme in plants was inferred by the work of Nandy and Ganguli (I%1) who reported that dehydroshikimate was a product in the incubation of erythrose-4-P and phosphoenolpyruvate with Phaseolus aureus extracts. Later the activity of the enzyme was found in several plant cell cultures and several plant sources (Minamikawaal., 1968 Balinsky and Davies, 1962). Balinsky and Davies (l%lc) partially purified the enzyme and studied its properties. There are reports that the enzyme occurs aggregated with shiki-mate dehydrogenase in a variety of plants including both monocots and dicots (Koshiba, 1978 Koshiba and Yoshida, 1976 Boudet and Lecussan, 1974 Boudet, et al., 1975). Monocots also contain a second unaggregated... 

In the next step of the sequence, 3-dehydroquinate (4) is dehydrated via a Z-elimination to produce 3-dehydroshiki-mate (5). This reaction is catalyzed by 3-dehydroquinate dehydratase. 3-Dehydroshikimate (5) is, in turn, reduced to shikimate (6) by 3-dehydroshikimate reductase, an enzyme that requires NADPH as a cofactor. Shikimate (6) is then converted to the 3-phosphate (7) by the action of shikimate kinase and ATP. 

Scheme 11.81. A proposed pathway from 3-dehydroquinate to 3-dehydroshikimate with 3-dehydroquinate dehydratase (EC 4.2.1.10) serving as the catalyzing enzyme.The suggestion of the mechanism follows Gourley, D. G. Shrive, A. K. Pohkarpov, I. Krell.T Coggins, J. R. Hawkins, A. R. Isaccs,N. W. Sawyer, L. Nat. Struct. Biol, 1999,6,521. ENZ = 3-dehydroquinate dehydratase (EC 4.2.1.10).

Adachi O, Ano Y, Toyama H, Matsushita K (2008b) A novel 3-dehydroquinate dehydratase catalyzing extracellular formation of 3-dehydroshikimate by oxidative fermentation of Gluconobacter oxydans IFO 3244. Biosci Biotechnol Biochem 72(6) 1475-1482. Epub 2008 Jun 7... 

Nishiknra-Imamura S, Matsntani M, Insmnphnn C, Vangnai AS, Toyama H, Yakushi T, Abe T, Adachi O, Matsushita K (2014) Overexpression of a type II 3-dehydioquinate dehydratase enhances the biotransfrmnation of quinate to 3-dehydroshikimate in Gluconobacter oxydans. Appl Mimtbiol Biotechnol 98(7) 2955-2963... 

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