DEAE ion-exchangers

Fig. 3.4. Procedure for recovery of small DNA fragments with DEAE ion-exchange paper.

DEAE ion-exchangers in 5 mM sodium phosphate buffer, pH 8.0, will either let the Fab pass or retain it lightly, so that the first protein to be desorbed by increasing the NaCl concentration in the buffer will be Fab. 

Econo-PacR Serum IgG Purification Kit Bio-Rad DEAE Ion exchange... 

Purification of Rabbit IgG Fraction from Rabbit Serum on DEAE Ion Exchange Column... 

Figure 1 Elution pattern by DEAE ion-exchange chromatography. 67 mg of solubilized proteins were deposited on the DEAE trisacryl M column (2 x 20 cm) equilibrated with the elution buffer (Tris/HCI 20 mM, 1 mM DTT, ImM benzamidine, pH 7,5) and eluaied with a gradient of 0 to 0,8 M NaCi in the same buffer.

A two-step purification procedure was used for the isolation of lethal protein toxin, Toxin-PC, including 50% ammonium sulphate precipitation followed by DEAE-ion exchange chromatography. Toxin-PC was homogenous in polyacrylamide gel electrophoresis with a SDS-molecular weight of near 15 kDa and devoid of glycoprotein moiety. As described in the results section, lethal Toxin-PC was highly active on isolated heart and nerve... 

Adenosine 5"-[P-thio]diphosphate tri-lithium salt [73536-95-5] M 461.1. Purified by ion-exchange chromatography on DEAE-Sephadex A-25 using gradient elution with 0.1-0.5M triethylammonium bicarbonate. [Biochem Biophys Acta 276 155 7972.]... 

Coiicin E (from E.coli) [11032-88-5], Purified by salt extraction of extracellular-bound colicin followed by salt fractionation and ion-exchange chromatography on a DEAE-Sephadex column, and then by CM-Sephadex column chromatography [Schwartz and Helinski J Biol Chem 246 6318 1971],... 

Proteoglycans (from cultured human muscle cells). Separated by ion-exchange HPLC using a Biogel TSK-DEAE 5-PW analytical column. [Harper et al. Anal Biochem 159 150 1986.]... 

As an example, consider the separation of the creatine kinase isoenzymes, MM, MB, and BB. Mercer has used classical ion-exchange chromatography (DEAE - Sephadex - A50) for the resolution of these three isoenzymes (44) To speed up the separation and ultimately to allow an automated analysis,... 

Figure 2 Ion-exchange chromatography on DEAE-Sepharose CL-6B (elution by acetate buffer pH 4.8) of (a) dialysed water-soluble pectins from extruded citrus fibres (SME = 250 kWh/t) and (b) dialysed acid extracted pectins from the same raw material, (empty symbols neutral sugars full symbols= galacturonic acids)...

The pectinase activity was found in void volume of the washing buffer while some contaminated protein was adsorbed to the ion exchanger (Figure 1. The activity was 100% recovered. Thoug i, the enzyme was not adsorbed to the DEAE-ceUulose, but it is useful since the some contaminated proteins coidd be separated. The enzyme was approx. 10 fold purified and its specific activity increased to 48.8 unit/mg protein (Table 1). 

Puma, P., Duffey, D., and Dawidczyk, P., U.S. Patent appl. 94,944, Purification of oligodeoxyribonucleotide phosphorothioates using DEAE-5PW anion ion-exchange chromatography and hydrophobic interaction chromatography, 1994. 

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