DEAE-agarose

Pack the exchanger (DEAE-agarose) into a suitable column (see Note 16) and equilibrate with 10-column volumes of 0.05 M Tris-HCl, pH 8.5. 

In order to obtain a fragment of catalase, it was immobilized by adsorption on various organic and inorganic carriers (such as aluminum oxide, diasorb DEAE, agarose), with further tryp-sine treatment. Aluminum wire or foil was used as the electrode. Such electrodes were selected because of their low price and inertness in relation to hydrogen peroxide. The binding agent between the electrode and biomimic was Pattex adhesive and 7.5% polyacrylamide gel. 

Ghethie, V., Schell, H. D. Electrophoresis and immunoelectrophoresis of proteins on DEAE-agarose gels, Rev. Roum. Biochim., 1967, 4, 179-184. 

Fractogel EMD DEAE, 10 cm x 2 cm ion-exchange column Matrex gel red A, agarose 5 % 10 cm x 2 cm affinity column lyophilizor. 

Transferrin (from human or bovine serum) [11096-37-0J Mr 80,000. Purified by affinity chromatography on phenyl-boronate agarose followed by DEAE-Sephacel chromatography. The product is free from haemopexin. [Cook et al. AB 149 349 1985 Aisen and Listowsky Annual Reviews of Biochem 49 357 1980). 

Diethylaminoethyl-agarose (e.g., DEAE-Sepharose Fast Flow). 

The strong interaction of dextran sulfates with cationic functions in porous support materials is exploited to create new highly charged surfaces for adsorption of proteins. It was revealed that new and strong ionic exchange resins are accessible by simple and rapid deposition of dextran sulfates on commercial DEAE- or MANAE-agarose. The material is characterised by an increased charge density on the porous surface of the support, which can perfectly bind protein material, as demonstrated in Fig. 15 [153]. 

NA45 DEAE anion-exchange membrane is a cellulose support containing diethyl aminoethyl (DEAE) functional groups. At low salt concentrations, DNA binds to DEAE -cellulose membranes [33,34]. Fragments of DNA are electrophoresed in a standard agarose gel until they resolve adequately. A... 

Figure 9.152 Profile of separation of relaxed DNA from supercoiled plasmid pBR329 on a DEAE-NPR column. The reaction was carried out with 1 unit of calf thymus topoisomerase I. Twenty microliters of the reaction mixture was applied on the column and eluted with a linear gradient of 0.5 to 0.65 M NaCl for 30 minutes. DNAs from peaks a and b were collected manually and analyzed by electrophoresis on a 1% agarose gel after ethanol precipitation. Inset O.C., open circular S.C., supercoiled. (From Onishi et al., 1993.)...

DEAE-Bio-Gel A Agarose 80-150 Diethylaminoethyl 2-9.5 Weak anion-exchanger... 

Gel electrophoresis can be used in semi-preparative separations of nucleic acids, with either agarose or acrylamide as matrix. The DNA or RNA band of interest is first located on the gel, usually by staining, and a piece of gel containing the band is excised. DNA can be extracted from the gel by various methods, either by electroelution in a dialysis bag, or by transfer of the DNA to a DEAE cellulose membrane. Alternatively, if the gel separation is carried out using low melting agarose, the piece of gel can be warmed to melt it, and the freed DNA can be adsorbed onto matrices such as DEAE cellulose, or fine glass beads, from which it can be released after... 

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