Data file list mode

The digital data from the flow cytometer are collected in list mode files that contain a record of all of the fluorescent events associated with each individual cell in a sample. For any individual cell, the number of events collected is dependent on the number of fluorochromes utilized in the analysis that bind to the cell, which can be amplified by increasing the variety/number of lasers utilized to excite the fluorochrome. These raw data can then be analyzed on-line or off-line by software provided by the manufacturer of... 

The number of files per disk is given as the number of samples (of 10,000 cells each) whose list mode data (4-parameter/ 1024-channel resolution) after acquisition can be stored to media of the indicated size. The capacity in bytes of the different media is representative but will vary with the formats of different computing systems. Similarly, different acquisition software will require more or less extra storage space for the housekeeping information that is stored with each sample. Prices of media are illustrative, but will vary considerably from place to place and over time. 

The data stored in FCS format are usually list mode data. As described above, this means that, in a four-parameter cytometer, four numbers are stored for each cell. A 10,000 cell data file will consist of a long list of 40,000 numbers, with each set of four numbers describing each cell in the sequence in which it passed through the laser beam. By retrieving the stored data, each cell can be analyzed again, and the intensity of each of the four signals for that cell will be known and can be correlated with each other or with the intensity of the four signals from any (or all) other cell(s). This type of list mode... 

The automatic homopolymer mode was convenient for calculations on many homopolymers, each represented by its own molecular data file containing both the atomic coordinates and the connectivity table, all under identical conditions. The directory containing the molecular data files was specified by the user. The program then automatically performed calculations on all requested molecular data files in that directory, in alphabetical order by their names. The output for each structure listed the calculated properties. 

To account for the presumably statistical distribution of Ni and Sn atoms in the 2(c) and 3(g) sites in this crystal structure, the initial distribution of atoms in the unit cell has been assumed as listed in Table 7.2. The initial profile and structural parameters are found in the input file for LHPM-Rietica on the CD, the file name is Ch7Ex01a.inp. Experimental diffraction data, collected on a Rigaku TTRAX rotating anode powder diffractometer using Cu Ka radiation in a continuous scan mode, are located on the CD in the file Ch7Ex01 CuKa.dat. 

Column 6—Failure Frequency Effects Probability. This column is optional. The failure frequency effects probability is a measure of the likelihood that the failure mode listed in column 2 will result in the system effect listed in column 4. This information is normally available only from lessons learned or other historical files and usually is not available for research and development projects. For many efforts, simple failure rates or mean times between failure data are substituted. 

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