Darzynkiewicz

Ewa Grudzien-Nogalska, )anusz Stepinski, )acek Jemielity, Joanna Zuberek, Ryszard Stolarski, Robert E. Rhoads, and Edward Darzynkiewicz... 

Grudzien, E., Kalek, M., Jemielity, J., Darzynkiewicz, E., and Rhoads, R. E. (2006). Differential inhibition of mRNA degradation pathways by novel cap analogs. J. Biol. Chem. 281, 1857-1867. 

Izaurralde, E., Lewis, J., McGuigan, C., Jankowska, M., Darzynkiewicz, E., and Mattaj, I. W. (1994). A nuclear cap binding protein complex involved in pre-mRNA splicing. Cell 78, 657-668. 

Niedzwiecka, A., Stepinski, J., Antosiewicz, J. M., Darzynkiewicz, E., and Stolarski, R. (2007). Biophysical approach to studying cap-eIF4E interaction by synthetic cap analogues. Methods Enzymol., 430, 209—246. 

Suggested references on fluorescent spectroscopy and the use of fluorescent probes include Lakowicz (1991, 1999) Bright (1988) Dewey (1991) McGown and Warner (1990) Ploem and Tanke (1987) Darzynkiewicz and Crissman (1990) Haugland (1991) and Waggoner (1990). 

Darzynkiewicz, Z., and Crissman, H.A. (1990) Flow cytometry. Meth. Cell Biol. 

Darzynkiewicz, Z., Crissman, H., and Jacobberger, J.W., Cytometry of the cell cycle cycling through history, Cytometry A, 58, 21, 2004. 

Gorczyca, W., Gong, J., and Darzynkiewicz, Z., Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick... 

Darzynkiewicz, Z. et al., Features of apoptotic cells measured by flow cytometry. Cytometry, 13, 795, 1992. 

Huang X, Okafuji M, Traganos F, Luther E, Holden E, Darzynkiewicz Z. Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by the DNA cross-linking agent cisplatin. Cytometry A. 2004 Apr 58(2) 99-110. 

Sharpless, T., Traganos, F., Darzynkiewicz, Z., Melamed, M.R., Flow cytometry Discrimination between single cells and cell aggregates by direct size measurement. Acta Cytol 19, 577-581 (1975). 

Kurose A, Tanaka T, Huang X, Halicka HD, Traganos F, Dai W, Darzynkiewicz Z (2005) Assessment of ATM phosphorylation on Ser-1981 induced by DNA topoisomerase I and II inhibitors in relation to Ser-139-histone H2AX phosphorylation, cell cycle phase, apoptosis. Cytometry A 68(1) 1—9... 

Although the concept of flow cytometry originated in studies of blood cells and tumor cells (for review, see Darzynkiewicz et ak, 2004), flow cytometric procedures are now used routinely in disciplines as diverse as immunology, the neurosciences, nutritional sciences, pharmacology, parasitology, and marine biology. This chapter offers an introduction to the theory and practice of analytical flow cytometry, with emphasis on applications in the neurosciences. 

The analysis of cell-cycle progression was one of the earliest applications of flow cytometry (for review, see Darzynkiewicz et al., 2004). In this assay, fluorescence signals from cells stained with DNA-binding fluorochromes are plotted as DNA content histograms that may be analyzed by using histogram deconvolution software to quantify cell-cycle phase distributions (Rabinovitch 1994). Fluorochromes that are useful for this purpose are the plasma membrane-impermeant DNA stains, propidium iodide (PI),... 

Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, et al. 1997. Cytometry in cell necrobiology Analysis of apoptosis and accidental cell death (necrosis). Cytometry 27 1-20. 

Darzynkiewicz Z, Crissman H, Jacobberger JW. 2004. Cytometry of the cell cycle Cycling through history. Cytometry 58A 21-32. 

Gong J, Traganos F, Darzynkiewicz Z. 1993. Simultaneous analysis of cell cycle kinetics at two different DNA ploidy levels based on DNA content and cyclin B measurements. 

Vereb G, Matko J, SzoUosi J (2009) Cytometry of fluorescence resonance energy transfer. In Darzynkiewicz Z, Robinson JP, Roederer M (eds) Essential cytometry methods. Elsevier, Oxford, pp 55-108... 

Bruno, S., Gorczyca, W., and Darzynkiewicz, Z. 1992. Effect of ionic strength in immunocyto-chemical detection of the proliferation associated nuclear antigens pi 20, PCNA and the protein reacting with Ki-67 antibody. Cytometry 73 496-501. 

Kapuscinski, J. and Darzynkiewicz, Z. (1984) Condensation of nucleic acids by intercalating cations. Proc. Natl. Acad. Sci. USA, 81,7368-7372. 

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