D-glycerol 3-phosphate

The structural unit of the phosphoglycerides is D-glycerol-3-phosphate, a derivative of the trivalent alcohol glycerol. The compounds glycerol and di-hydroxy-acetone were detectable in the Murchison meteorite. This does seem noteworthy as these biogenic compounds are not in the products of the prebiotic soup. What is the mechanism of lipid formation in meteorites This question has not been answered. 

D-glycerol-3-phosphate (s -glycerol-3-phosphate) (47X which is used in phospholipid manufacture, is another synthon whose production requires the recycling of a co-factor. It is synthesized from glycerol and ATP, with... 

Glycerol L-Glycerol 3-phosphate oro-glycerol 1-phosphate sn-glycerol 3-phosphate L-Glycerol 1-phosphate or D-glycerol 3-phosphate sn-glycerol 1-phosphate... 

Adams, P.R., Harrison, R., and Inch, T.D., Dehydrogenation of a phosphonate substrate analogue by glycerol 3-phosphate dehydrogenase, Biochem.., 141, 729, 1974. 

Auer, M, Kim, M. J., Lemieux, M. J., Villa, A., Song, J., Li, X.-D. and Wang D.-N. (2001). High-yield expression and functional analysis of Escherichia coli glycerol-3-phosphate transporter, Biochemistry, 40, 6628-6635. 

Oxidation by raising the pH (to 7.5) phytase activity is switched off hydrolysis is prevented. Oxidation of L-glycerol-3-phosphate to DHAP by GPO at 55% glycerol (v/v) is quantitative. Catalase is added to suppress the build-up of hydrogen peroxide. The D-isomer is converted back into glycerol and phosphate in the last step. 

Zhu, M. M. Lawman, P. D. Cameron, D. C. Improving 1,3-propanediol production from glycerol in a metabolically engineered E.coli by reducing accumulation of glycerol-3-phosphate, Biotechnol. Prog., 2002, 18, 694-699. 

This enzyme [EC 4.2.1.19] catalyzes the conversion of D-eryrfiro-l-(imidazol-4-yl)glycerol 3-phosphate to 3-(imidazol-4-yl)-2-oxopropyl phosphate and water. 

This enzyme [EC 4.1.1.48] catalyzes the conversion of l-(2-carboxyphenylamino)-l-deoxy-D-ribulose 5-phos-phate to generate l-(indol-3-yl)glycerol 3-phosphate, carbon dioxide, and water. In some organisms, this enzyme is part of a multifunctional protein together with one or more components of the system for the biosynthesis of tryptophan (/.e., anthranilate synthase, anthranilate phosphoribosyltransferase, tryptophan synthase, and phosphoribosylanthranilate isomerase). 

MONOACYLGLYCERIDE LIPASE 1-Acyl-sn-glycerol 3-phosphate, LYSOPHOSPHOLIPASE D ACYLGLYCERON E-PHOSPHATE REDUCTASE... 

S glycerol 3-phosphate -h D-glucose <1> (<1> involved in anaerobic metabolism of sugars in bloodstream trypanosomes [1]) (Reversibility <1>... 

As depicted in Figure 6.8 the stability screening was based on DERA activity assay, the retro-aldol reaction of 2-deoxy-D-ribose 5-phosphate to acetaldehyde and D-glyceraldehyde 3-phosphate. D-glyceraldehyde 3-phosphate is further converted by the auxiliary enzymes triose phosphate isomerase and glycerol phosphate dehydrogenase. As the latter reaction consumes NADH it can be measured spectro-pho to metrically by the decrease in absorbance at 340 nm. 

Fig. 17. Nucleotidyltransferase substrate screening assay (NUSSA). A Nucleotide sugar-synthesizing nucleotidyltransferase (EC 2.7.7), B PPrdependent phosphofructokinase (EC 2.7.2.90), C Aldolase (EC 4.1.2.13), D Triose-phosphate isomerase (EC 5.3.1.1), E Glycerol-3-phosphate dehydrogenase (EC 1.1.1.8) [336]...

Glucose (Glc) is taken up and phosphorylated into glucose-6-phosphate (Glc6P), with consumption of ATP. Isomerization and phosphorylation afford fructose-l,6-bisphosphate (Frul,6P2), which is cleaved into two triose molecules D-glyceraldehyde-3-phosphate (GA3P) and dihydroxyacetone monophosphate (DHAP). These are equilibrated by triose phosphate isomerase as only GA3P is metabolized further, except approximately 5 mol% of DHAP that leaks out of the pathway via reduction to glycerol, which is excreted as a side-product. 

Fig. 8.4 The glycolysis pathway in Saccharo-myces and the biosynthesis of ethanol and glycerol. Compounds DHAP, dihydroxyl-acetone monophosphate Fru6P, D-fructose-6-phosphate Frul,6P2, D-fructose-1,6-bisphosphate GA3P, D-glyceraldehyde-3-phosphate GL3P, sn-glycerol-3-phosphate GLAP2, phosphoglycerate-3-phosphate ...

---