Cyclodextrins supercritical fluid chromatography

TABLE 11 The Enantiomeric Resolution of Racemic Compounds on Cyclodextrin-Based CSPs by Means of Subcritical and Supercritical Fluid Chromatography... 

In addition to high-performance liquid chromatography (HPLC), the chiral resolution using CMPAs was also carried out by supercritical fluid chromatography (SFC) [91] and capillary electrochromatography (CEC) [92-98]. Salvador et al. [91] used dimethylated /1-cyclodextrin as the mobile phase additive on porous graphite carbon as the solid phase for the chiral resolution of tofizopam, warfarin, a benzoxazine derivative, lorazepam, flurbiprofen, temazepam, chlorthalidone, and methyl phehydantoin by SFC. The authors also studied the effect of the concentration of dimethylated /1-cyclodextrin, the concentration of the mobile phase, the nature of polar modifiers, outlet pressure, and the column temperature on the chiral resolution. 

M. Jung and V. Schurig, Extending the scope of enantiomer separation by capillary supercritical fluid chromatography on immobilized polysiloxane anchored permethyl-B-cyclodextrin (Chirasil-Dex), J. High Resolut. Chromstogr., 76 215 (1993). 

Recent developments in separation science are covered from the standpoint of their impact on various modes of chromatography. Chromatographic applications of cyclodextrin and phenylboronic acid stationary phases are discussed. The renaissance in electrophoresis has given risen to high-resolution, two-dimensional gel electrophoresis. The basic methodology and recent advances are provided for studies on protein separations. Finally, the combination of capillary supercritical fluid chromatography and mass... 

K.L. Williams, L.C. Sander and S.A. Wise, Comparison of Liquid and Supercritical Fluid Chromatography Using Naphthylethylcarbamoylated- 3-cyclodextrin Chiral Stationary Phases, J. Chromatogr. A, 746(1996)91. 

Armstrong, D. Tang, Y. Ward, T. Nichols, M. Derivatized cyclodextrins immobilized on fused-silica capillaries for enantiomeric separations via capillary electrophoresis gas chromatography, or supercritical fluid chromatography. Anal. Chem. 1993, (55, 1114-1117. 

Capillary zone electrophoresis with UV detection has been used to separate ergometrine, ergotamine, and their epimers [30]. The addition of cyclodextrins in phosphate buffer at pH 2.5 was required to resolve five ergopeptine alkaloids and their epimers. Supercritical fluid chromatography has also been used with UV detection at 280 nm or mass spectrometry to identify clavine alkaloids in extracts of Claviceps purpurea. 

Wang RQ, Ong TT, Ng SC (2008) Synthesis of cationic P-cyclodextrin derivatives and their applications as chiral stationary phases for high-performance liquid chromatography and supercritical fluid chromatography. J Chromatogr A 1203 185-192... 

ASE, accelerated solvent extraction HPGPC, high-performance gel permeation chromatography OSUA, oligomers of sodium undecylenic acid SEE, supercritical fluid extraction SDpCD, sulfobutyl ether p-cyclodextrin MpCD, methyl p-cyclodextrin PFOS , perfluorooctanic sulfate DMSO, dimethyl sulfoxide THA+, tetrahexylammonium SPME, solid-phase microextraction STDC, sodium taurodeoxycholate LIE, laser-induced fluorescence LLE, liquid-liquid extraction. 

Alcohol reflux extraction, ultrasound assisted extraction, Soxhlet extraction and supercritical fluid extraction (SPE) are the main teehniques used to isolate triterpenoids from Cornus spp. fimits [Zhao et al., 2005], Thin layer chromatography (TEC), HPLC and cyclodextrin-modified micellar eleetrokinetie ehromatography (CD-MEKC) have also been apphed for separation and determination of these three triterpenic acids in Iriiits [Zhang et al., 2005 Wang et al., 2008]. The isolated triterpenie compounds and their glycosides were identified by speetroscopie analysis NMR, IR and MS. 

---