Cultured excise roots, effect

In the meantime, the formation of the main alkaloids in C. ipecacuanha under a variety of conditions has been extensively investigated emetine (1) in callus cultures (49) and under the effects of L-tyrosine supplementation (5t)) emetine (1) and cephaeline (2) in Panamanian ipecac (57), in Nicaraguan ipecac (52), in regenerates obtained by clonal propagation (53,54), in tissue cultures (55) and under the effects of exogenous feeding of shikimic acid and L-phenylalanine (55), in cell suspension and excised root cultures (57), in adventitious root cultures (58), and in callus cultures (56,59) and the effects of age and electrokinetic potential (60) ipecoside (7) in the roots (61) and the effect oi Azotobacter, leaf mold, and farmyard manure on alkaloid content (62). In addition, micropropagation systems for C. ipecacuanha have been developed (63-65). 

Figure 1. Effect of epiBR on growth of cultured excised tomato roots after 7 days. Inocula were 10 mm with no laterals.

LINEHAN D.J. 1976. Some effects of a fulvic acid component of soil organic matter on the growth of cultured excised tomato roots. Soil Biology and Biochemistry, 511-517. 

Walker, S.J., Llewellyn, G.C., and Lillehoj, E.B. (1984). Uptake and subcellular distribution of aflatoxin B by excised cultured soybean roots and toxin effects on root elongation. Environ. Exp. Bot., 24, 113-122. 

We investigated the effect of inoculum conditions such as the part, number, and length of root tips age of the hairy roots and size of inoculant on the growth and metabolite biosynthesis of P. ginseng C. A. Meyer hairy root culture. The growth of P. ginseng hairy roots in shake-flask cultures was found to vary depending on the inoculum conditions. The end part, whose apical meristem of root tip had been excised prior to inoculation,... 

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