Culture media, agar

See also Biotransformations Microbial oxidations Microbial reductions applications of, 76 396-399 biocatalyst selection in, 76 404-409 biocatalysts in, 76 409-414 for drug metabolite production, 76 398-399 further advances in, 76 414 in hydrolysis, 76 400-401 multiphase reactions in, 777 412-414 scale-up of, 76 414 systematic studies of, 76 398 technique overview for, 76 403-414 timing of substrate additions in, 76 411-412 uses for, 777 400-403 Microbial waxes, 26 203 Microbiocides, triorganotins as, 24 817 Microbiological culture media, agar in, 73 68... 

Surface plating Several methods are now in use that lead to the growth of all colonies on the surface of the culture medium - agar plate, dry film, hydro-phobic grid membrane filter (HGMF), etc. 

Nfthr-. nutritive, nutrient, alimentary, -agar, m. nutrient agar, -aquivalent, n. nutritive equivalent or value, -biatt, n. (Bot.) storage leaf, -boden, m. nutrient medium, nutrient substrate, culture medium, -bouillon, -briUie,/. nutrient broth, nahren, v.t. feed nourish support. — nah-rend, p.a. nourishing, nutritive, nutritious, nutrient. 

E.coli recA y.luxCDABE strain were grown for 16-18 hours at 37°C in LB-broth in the presence of 20 pg/ ml of ampicillin. Immediately before the experiment the culture was diluted 1 20 by fresh culture medium and incubated until early log-phase. The grown biomass was mixed with AR solutions in final concentrations of ICfs, ICH n ICfs M, with used for their dilution with distilled water (control) and incubated for 60 minutes. The luminescence intensity of UV-irradiated E.coli recA lux and intact specimens were registered by plate bioluminometer LM OIT (Immimotech, Czech Rep.) in a real time. The number of viable cells was determined from the colony-forming units (CFU) on a surface of a LB-agar after the subsequent incubation within 24 hours at 37 °C. A quantitative estimation of an induction of the SOS-system calculated on formula... 

Siderophore production by rhizosphere bacteria that are culturable on agar media has been estimated by plating out colonies on an indicator agar medium containing chrome azurol, a weak iron-chelating complex that changes color upon... 

Algal cultures in exponential phase are used for inoculating Petri dishes containing agarized culture medium... 

CULTURE MEDIUM GC AGAR BASE DEHYDRATED 0.25 POUNDS 6550009355870 BT 21.41 ... 

In order to obtain pure cultures, the crude culture which contained Chromatium species was serially diluted in this modified medium as suggested by van Niel ( 1). This was repeated several times to ensure the purity of the culture. The agar shake method was not used as the large photosynthetic bacteria fail to grow in agar shake tubes ( 1). In pure culture the Chromatium species did not grow as well as in the mixed culture situation. The culture was not as dense or as rich in colour as the crude cultures. 

Cotton swabs or RODAC plate (nutrient agar culture medium)... 

Agar-agar is used for the prepn of glue, as a culture medium and as a binding agent in some propellant and expl compositions (Ref 1)... 

The method is based on the perturbation of the respiratory activity of yeast, Saccharomyces cerevisiae, immobilized on an agar gel containing the culture medium (i.e., agarized medium ), by the toxic test substance. Glucose is used as substrate while the substances tested consist of several metallic ions, phenols and cationic or anionic surfactants, pesticides and other toxics. 

The production of extracellular polysaccharides was done using a culture medium similar to YMA, supplemented with manganese ions (0.12 g/L of MnCl2-4H20) and omitting agar. The pH of this medium was adjusted to 7.0 by the addition of NaOH (50%, w/v). 

To look for PRP-resistant mutants, plate an undiluted sample of each mutagenized culture on agar medium containing the PRP at the selected concentration (see Subheading 3.1). Incubate all the plates at 37°C and store the cultures at 4°C. 

Agar Culture Medium Add 6.0 g of agar to 500 mL of Liquid Culture Medium, and heat with stirring on a steam bath until the agar dissolves. Add approximately 10-mL portions of the hot solution to test tubes, cover to prevent contamination, sterilize by heating in an autoclave at 121 ° for 15 min, and cool tubes in an upright position to keep color formation to a minimum. Store at 10° in the dark. 

The Trp+ colonies obtained from tubes 2 and 3 may be too numerous to count. For this reason you must confirm the number of transformed (Trp+) cells in tubes 2 and 3 by transferring a 0.1-ml sample from each of these tubes into separate marked tubes containing 9.9 ml of sterile saline. Mix these solutions thoroughly and plate a 0.1-ml sample of the 100-fold diluted samples on separate marked plates containing minimal medium agar. Save the diluted culture samples for later use. 

Plantlet cultured on agar medium and later transferred to soil... 

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