Cryogenic-transmission electrons microscopy

Portnaya, I., Cogan, U., Livney, Y.D., Ramon, O., Shimoni, K., Rosenberg, M., Danino, D. (2006). Micellization of bovine p-casein studied by isothermal titration microcalorimetry and cryogenic transmission electron microscopy. Journal of Agricultural and Food Chemistry, 54, 5555-5561. 

Zidovska A, Ewert KK, Quispe J, Carragher B, Potter CS, Safinya CR (2009) The effect of salt and pH on block liposomes studied by cryogenic transmission electron microscopy. Biochim Biophys ActaBiomembr 1788 1869-1876... 

These structures were proven by various methods. Cryogenic transmission electron microscopy confirmed the existence of the supermicelles in solution (Fig. 22), while scanning electron microscopy (Fig. 23) and scanning force microscopy could prove larger particles with a non-uniform size of still unknown nature besides the supermicelles and unimers. 

Won Y-Y, Brannan AK, Davis HT et al (2002) Cryogenic transmission electron microscopy (cryo-TEM) of micelles and vesicles formed in water by poly(ethylene oxide)-based block copolymers. J Phys Chem B 106 3354-3364... 

It is possible to observe the morphologies of the cationic PMETAI stars in solution by cryogenic transmission electron microscopy (cryo-TEM). Figure 1 clearly shows the distinct and well-separated structures in the vitrified solution. In some cases it is even possible to see the silsesquioxane core [28],... 

Kesselman, E., Talmon, Y., Bang, J. et al. (2005) Cryogenic transmission electron microscopy imaging of vesicles formed by a polystyrene-polyisoprene diblock copolymer. Macromolecules, 38 (16), 6779-6781. 

Cryogenic transmission electron microscopy (Cryo-TEM) or cryogenic high resolution transmission electron microscopy (Cryo-HRTEM) involves the observation of samples of vitrified macromolecules. 

H. Cui, T. K. Hodgdon, E. W. Kaler, L. Abezgauz, D. Danino, M. Lubovsky, Y. Talmon, D. J. Pochan, Elucidating the assembled structure of amphiphUes in solution via cryogenic transmission electron microscopy. Soft Matter, 2007, 3, 945—955. 

Figure 1 Cryogenic transmission electron microscopy and optical microscopy with controlled environment vitrification. (From Ref. 9.)...

Danino, D., Bemheim-Groswasser, A., and Talmon, Y, Digital cryogenic transmission electron microscopy an advanced tool for direct imaging of complex fluids. Colloids Surfaces A, 183-185, 113, 2001. 

In life sciences research a huge variety of (high dimensional) images is generated, with many new types of microscopy (confocal, two-photon, cryogenic transmission electron microscopy, etc.) and dedicated (bio-) medical small animal scanners (micro-CT, mini PET, mouse-MRl, etc.). The research on molecular imaging and molecular medicine is still primarily done in small animal models. 

As a complementary technique to SAXS, the cryogenic Transmission Electron Microscopy (cryo-TEM) enables the visualization of the shape of the dispersed particles and their internal nanostmctures. The right side of Figure 2... 

The various topologies of DNA (supercoiled, circular, linear) can be discriminated by various methods such as electrophoresis and by microscopy techniques such as electron microscopy (EM) [36], cryogenic transmission electron microscopy (cryo-TEM) [37], and atomic force microscopy (AFM) [38]. 

Vesicles can be characterized with the help of a range of physicochemical methods. Microscopy is an essential tool in all investigations of vesicles. MLVs and GUVs can be observed in real time by optical and fluorescence microscopy. LUVs and SUVs are best observed by transmission electron microscopy (TEM), ideally by stain-free methods such as cryogenic transmission electron microscopy (cryo-TEM), which can easily resolve the individual membrane secluding each vesicle. Depending on the stability of vesicles in the absence of solvent, vesicles can... 

Cryogenic transmission electron microscopy Observe morphology of each phase The samples in each phase were frozen to form a thin ice film, which was observed by electron microscopy... 

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