Cranberries phenolic concentrations

RP-HPLC has been employed for the determination of flavonoids and other phenolic compounds in cranberry juice. The neutral and acidic analytes were preconcentrated octadecyl silica SPE cartridges conditioned with distilled water (neutral analytes) or with 0.01 M HC1 (acidic compounds). Hydrolysis of samples was carried out in aqueous methanol solution acidified with 6 M HC1 at 35°C for 16h. Chromatographic separation was performed in an ODS column (150 X 4.6mm i.d. particle size 5/.an). Solvents A and B were water-acetic acid (97 3, v/v) and methanol, respectively. The gradient started with 0 per cent B (flow rate, 0.9 ml/min), reached 10 per cent B in lQmin (flowrate, 1.0 ml/min) and increased to 70 per cent B in 40min (flowrate, 1.0 ml/min). Analytes were detected at 280 and 360 nm. Some typical chromatograms are presented in Fig. 2.71. The concentrations of flavonoids and phenolic acids are compiled in Table 2.69. It was stated that the SPE-HPLC procedure makes possible the simultaneous determination of phenolic compounds and flavonoids, therefore, it can be employed for the measurement of these classes of analytes in other fruit juices [188],... 

CONCENTRATIONS OF FLAVONOIDS AND PHENOLIC ACIDS IN CRANBERRY JUICE SAMPLES... 

Recent studies are starting to build evidence that these foods help to improve the antioxidant potential in vivo. For example, Pedersen and associates [51] have shown that consumption of cranberry juice was able to increase the plasma antioxidant capacity of healthy female volunteers, by determining a 30% increase in vitamin C and a small but significant increase in total phenols plasma concentration. 

The total phenolic content increase after soymilk fermentation of the LP2 strain with the cranberry-chitosan oligosaccharide mixture addition at various concentrations was further correlated with H. pylori inhibition (Apostolidis et al. 2011). This finding is supported by previous reports indicating the potential of phenolic-containing plant extracts for... 

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