Corynebacterium ammoniagenes

The above reaction was known many years ago (Komberg, 1950 Wang and Kaplan, 1954) and found both in procaryotes and eucaryotes. In Brevibacterium ammoni-agenes (Murata et al., 1979), Micrococcus luteus and Corynebacterium ammoniagenes (Fillipovich et al., 2000) phosphorylation of NAD using PolyP as a phosphate donor was revealed ... 

First they developed the process for production of 5 -GMP by hybridizing the XMP fermentation of Corynebacterium ammoniagenes with the energy-requiring amination reaction catalyzed by GMP synthase [28] ... 

The rib operon of Corynebacterium ammoniagenes is structured as the rib operons of B. subtilis or C. glutamicum. Purine analogue-resistant C. ammoniagenes mutants, which were transformed with plasmids encoding the... 

Corynebacterium ammoniagenes DN510 E coli NM522/pNT25/pNT32... 

Fujio, T., and A. Maruyama. 1997. Enzymatic production of pyrimidine nucleotides using Corynebacterium ammoniagenes cells and recombinant Escherichia coli cells enzymatic production of CDP-choline from orotic acid and choline chloride (part I). Biosci. Biotechnol. Biochem. 61 956-959. 

The method of synthesis ATP from adenine using Corynebacterium ammoniagenes cells as enzyme sources, which was free from enzyme purification, has been developed (33). In this system, cells were permeabilized by the treatment with surfactant and organic solvent. Phosphoribosylpyrophosphate and ATP were supplied by the metabolism of the cells from glucose. In the same manner, CDP-choline was produced by the... 

Hogbom M, Hnqne Y, Sjoberg B-M, Nordlrmd P. 2002. Crystal structure of the di-iron/radical protein of ribonucleotide reductase from Corynebacterium ammoniagenes. 

Koizumi, S., Yonetani, Y., Maruyama, A., and Teshiba, S. (2000) Production of riboflavin by metabohcaUy engineered Corynebacterium ammoniagenes. Appl Microbiol Biotechnol 53, 674-679. 

The first bona fide MnRNR, isolated from Corynebacterium (formerly Brevibacterium) ammoniagenes, was reported about 10 years ago [122], The MnRNR R1 protein is monomeric (dimeric in E. coli) [123]. A dinuclear Mnm—0—Mnm unit was believed to be present at its active site (cf. oxidized E. coli RNR), and its sensitivity to hydroxyurea (a radical scavenger) suggested a mechanism similar to that of its iron counterpart. However, no tyrosyl radical EPR signal was observed from the MnRNR, casting doubts about the redox role of the manganese center [118], Very recently, however, a stable EPR signal, which is inhibited by hydroxyurea and correlates directly with enzymatic activity, has been detected [124],... 

Corynebacterium utamicum transketolase mutants, which contained a plasmid encoding the C. ammoniagenes rib operon, demonstrated superior riboflavin production over transketolase wild-type host strains provided with the same plasmid. The experiments were carried out in test tubes reaching 41 and 108 mg 1 riboflavin with the transketolase wild type and mutant strain, respectively. It is not clear to which extent these results are relevant for industrial high-intensity fed-batch fermentation runs. 

Other type I PanK enzymes that have been characterized are the M. tuberculosis enzyme, of which the crystal structure has been determined, and the enzyme from Corynebacterium (previously Brevibacterium) ammoniagenes ° an industrially relevant organism. 

Mycobacterium smegmatis, Corynebacterium diphtheriae, Streptomyces coelicolor, Brevibacterium ammoniagenes. 

Isol. from Brevibacterium ammoniagenes. Oxidative stress metab. of various nocar-dioform bacteria inch Corynebacterium and Micrococcus spp. Oxidative stress agent. 

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