Confocal scanning laser microscopy cell culture

Davis, W. P Janssen, I. M. W Mossman, B. T. Taatjes, D. J. Simultaneous triple fluorescence detection of mRNA localization, nuclear DNA, and apoptosis in cultured cells using confocal scanning laser microscopy. Histochem. Cell Biol. 1997, 108, 307-311. 

The cellular uptake of these 3LNPs was observed using confocal scanning laser fluorescence microscopy. SKOV-3 ovarian cancer cells were cultured with... 

Rothen-Rutishauser, B., Braun, A., Gunthert, M., and Wunderli-Allenspach, H., Formation of multilayers in the Caco-2 cell culture model a confocal laser scanning microscopy study, Pharm. Res., 17, 460, 2000. 

We thank Dr. Jerome and Dr. Nahde for helping with the cell culture and animal experiments and Dr. Merdan for the confocal laser scanning microscopy experiments. We would also like to thank vectron therapeutics... 

Figure 1.6 Scanning electron microscopy images of the surface of (a) as-machined and (b) Ti-coated PEEK. Confocal laser scanning microscopy images of MC3T3-E1 cells cultured on (c) as-machined and (d) Ti-coated PEEK for 3 h.

Classically, laser scanning confocal microscopy has been used to investigate Ca2+ waves. For relatively flat, cultured cells, less technically demanding epifluorescence... 

Chandra, S. Kable, E. P Morrison, G. H. Webb, W. W. Calcium sequestration in the Golgi apparatus of cultured manunalian cells revealed by laser scanning confocal microscopy and ion microscopy. J. Cell Sci. 1991, 100, 747-752. 

Transfection techniques in vitro and ex vivo (organotypic cultures) offer an array of possibilities to investigate the consequence) s) of the introduction of foreign nucleic acids (DNA but also RNA) and or other biologically active molecules into neurons, and to combine observations with immunocytochemistry. In particular, a wide number of fluorescent reporter proteins (FRPs) can be employed for multicolor fluorescence imaging. Here we present a series of protocols for in vitro and ex vivo transfection of DNA or RNA sequences into cerebellar neuron cultures and organotypic slices based on the use of plasmid vectors and multicolor laser scanning confocal microscopy. These protocols allow analysis of live transfected cells, and, after fixation, correlative neurochemical studies. 

---