Confocal polymers

Ribbe A E 1997 Laser scanning confocal microscopy in polymer science Trends Polym. Sc/. 5 333-7 Oliveira M J and Hemsiey D A 1996 Optical microscopy of polymers Sonderb. Prakt. Metallogr. 27 13-22 Nie Sh and Zare R N 1997 Optical detection of single molecules Ann. Rev. Biophys. Biomol. Struct. 26 567-96 Masters B R 1994 Confocal redox imaging of cells Adv. Mol. Cell Biol. 8 1-19... 

Schrof W, Klingler J, Heckmann W and Horn D 1998 Confocal fluorescence and Raman microscopy in industrial research Colloid Polym. Sc/. 276 577-88... 

Bhawalkar J D, Swiatkiewicz J, Pan S J, Samarabandu J K, Liou W S, He G S, Berezney R, Cheng P C and Prasad P N 1996 Three-dimensional laser scanning two-photon fluorescence confocal microscopy of polymer materials using a new, efficient upconverting fluorophore Scanning 18 562-6... 

Ling X, Pritzker M D, Byerley J J and Burns C M 1998 Confocal scanning laser microscopy of polymer coatings J. Appl. Polym. Sc/. 67 149-58... 

FIG. 9 Confocal laser scanning micrograph of a hollow polymer capsule. The polymer capsule was obtained from polymer multilayer-templated FDA microcrystals after removal of the colloidal core. The FDA microcrystals were coated with SDS and 11 polyelectrolyte layers [(PAH/PSS)3/PAH/ (PSS/PAH-FITC)2]. (PAH-FITC = PAH labeled with fluorescein isothiocyanate.) The microcrystal core was removed by exposure of the coated microcrystals to ethanol, causing solubilization of FDA. 

Kim, H.-B., Hayashi, M., Nakatani, K., Kitamura, N., Sasaki, K., Hotta, J.-I., and Masuhara, H., In situ measurements of ion exchange processes in single polymer particles laser trapping microspectroscopy and confocal fluorescence microspectroscopy, Anal. Chem., 68, 409, 1996. 

Bowman R.D., Kneas K.A., Demas J.N., Periasamy A., Conventional, Confocal And Two-Photon Fluorescence Microscopy Investigations of Polymer-Supported Oxygen Sensors, J. Microscopy 2003 211 112-120. 

Confocal fluorescence microscopy has been extensively used in cell biology. Single living cells can indeed be studied by this technique visualization of organelles, distribution of electrical potential, pH imaging, Ca2+ imaging, etc. (Lemasters, 1996). Interesting applications in chemistry have also been reported in the fields of colloids, liquid crystals and polymer blends. 

Confocal fluorescence microscopy images in a polymer film of a second-generation polyphenylene dendrimer with eight perylenemonoimide functions at the periphery 49 and a model compound bearing one single perylenemonoimide 50 reveal that the dendrimer exhibits a three- to fourfold intensity and an obvious blinking in comparison to the model compound which shows only uniform spots with less dynamics (Scheme 19) [69]. 

Fig. 23 Confocal laser scanning microscopic image of rhodamine-labeled SiP coated with PMMA brush The diameter of silica particle core is 230 nm, and the Mn of the graft polymer is 256000...

Owing to the simphcity and versatility of surface-initiated ATRP, the above-mentioned AuNP work may be extended to other particles for their two- or three-dimensionally ordered assemblies with a wide controllabiUty of lattice parameters. In fact, a dispersion of monodisperse SiPs coated with high-density PMMA brushes showed an iridescent color, in organic solvents (e.g., toluene), suggesting the formation of a colloidal crystal [108]. To clarify this phenomenon, the direct observation of the concentrated dispersion of a rhodamine-labeled SiP coated with a high-density polymer brush was carried out by confocal laser scanning microscopy. As shown in Fig. 23, the experiment revealed that the hybrid particles formed a wide range of three-dimensional array with a periodic structure. This will open up a new route to the fabrication of colloidal crystals. 

Schafer, T., Di Paolo, R. E., Franco R.,and Crespo, J. G., Elucidating interactions of ionic liquids with polymer films using confocal Raman spectroscopy, Chem. Commun., 2594-2596,2005. 

FIGURE 4.6 Quantification of fluorescence intensity (expressed in arbitrary units per square micron) of the confocal fluorescence images of SW-480 cells after incubation with various polysaccharide containing HPMA copolymer-FITC conjugates and the control polymer (P-F) after 24 h at 37°C. (From David, A. et al., Pharm. Res., 19, 1114, 2002. With permission.)... 

P 66] Equal streams of 1 mM solutions of fluorescein-labeled polymer (polyethylen-imine, molecular weight 500 000) in water-glycerol mixtures (0 and 80% glycerol) and a clear solution were injected into the channel [44], The flow was achieved by compressed air at constant pressure. Imaging was achieved by applying a confocal fluorescence microscope. 

---