Confocal micrograph

Figure 2.42 Micro mixer geometry with staggered groove structures on the bottom wall, as considered in [137], The top of the figure shows a schematic view of the channel cross-section with the vortices induced by the grooves. At the bottom, confocal micrographs showing the distribution of two liquids over the cross-section are displayed.

Figure 13.6 (a) Confocal micrograph of a circularly self-spreading lipid monolayer. A rhodamine-labeled lipid is doped to visualize the spreading behavior, (b) A schematic illustration of the front edge of the self-spreading lipid monolayer [51]. 

Figure 6.15 Confocal micrographs (100 pm x 100 pm) showing the microstructure of acid-induced sodium caseinate gels (3 w /v%) (a) without any sucrose at pH = 5.2 (b) in the presence of 60 w/v% sucrose at pH = 4.9. Reproduced from Belyakova el al. (2003) with permission.

Figure 6.21 Confocal micrographs of samples of 0.5 wt% WPC emulsions containing CaCl2 added prior to emulsification A, no added CaCl2 B, 3 mM C, 5 mM D, 10 mM. Scale bars correspond to 10 pm. Reproduced from Ye and Singh (2000b) witli permission.

Figure 8.4 Effect of replacing small fraction of p-lactoglobulin by sodium caseinate on microstructure of concentrated oil-in-w7ater emulsion (45 vol% oil, 3 wt% total protein, pH = 6.8, ionic strength = 0.03 M) heated for 6 min at 90 °C. Confocal micrographs were obtained with Rhodamine B as fluorescent protein stain (a) emulsion contains 3 wt% p-lactoglobulin (b) emulsion contains 2.85 wt% p-lactoglobulin + 0.15 wt% caseinate. Scale bar = 20 pm. Reproduced from Parkinson and Dickinson (2004) with permission.

As w ell as lateral heterogeneity in mixed protein layers, there is also the possibility of segregation of biopolymer components perpendicular to the interface, z.e., bilayer formation (Dickinson, 1995, 2009). Let us consider the case of an interface containing casein and whey protein in an emulsion system. The images in Figure 8.4 are confocal micrographs... 

Laser scanning confocal micrograph of chromosomes at metaphase. Courtesy of Tom Moninger... 

Figure 8.2 Confocal micrograph of a sample from the rag layer shown in Figure 9.1. The oil is the bright phase it exhibits structures typical of gels (black arrows) which are often found in oxidized crude oil. This rag layer contained between 20 to 30% oil, which represents a significant potential loss of recovery. The water is the dark phase it exhibits aggregates of clay particles and emulsified oil (white arrow). Bar = 50 Xm. From Mikula and Munoz [68]. Copyright 2000, Cambridge University Press.

Fig. 4.4.3. Covalent labeling of nuclear targeted wl60hAGT-NLS3 in AGT-deficient CHO cells. Confocal micrographs A-C show overlays of transmission and fluorescence channels (exc 488 nm). The size bar in A-C corresponds to 10 pm. Confocal micrographs (A-C) illustrate the time course of the labeling of transiently expressed wl60hAGT-NLS3 with BGAF in AGT-deficient CHO cells. (A) AGT-deficient CHO...

Fig. 3. (A) Far-field confocal micrograph (35 pm x 35 pm) of a mica-supported DPPC monolayer showing LE-LC phase coexistence, deposited at a surface pressure of 9mN/m. (B) Atomic force micrograph of the film depicted in (A). Bright features denote topographically higher substructure of the film. (C) Near-held fluorescence image of the him shown in (A). (D) Near-held topology image collected simultaneously with the image depicted in (C). Reproduced with permission from Ref. [18]. Copyright 1998 Biophysical Society.

Figure 10 Confocal micrographs that show the diminishing fluorescein concentration (fluorescence intensity) in the SC as a function of ultrasound exposure. — heat-stripped human cadaver skin loaded with fluorescein. Right—skin exposed to ultrasound for 30 min. (Reproduced from Ref 75.)...

Fig. 18 Confocal micrographs of 24 h hypoxic H9C2 cardiocytes treated with rhodamine labeled IL (B) or rhodamine labeled PL (A). The micrographs are shown in pseudocolors. Cells treated with IL showed retention of membrane integrity and cell morphology (A). Liposomes represented as yellow colored regions are also discernable on the cells. Cells treated with PL showed presence of only dead cells with only a few cells having non-specifically attached PL (B). (From Ref...

Figure 3-17 Confocal micrograph showing a forty-micrometer stereo slice in a 90-pm thick section of mouse cerebellum. Courtesy of A. Boyde.

Figure 1.48 Confocal micrographs of polyurethane foam with labeled particulates (a) highlights of particulates and (b) locations of particulates on the foam surfaces. The scale bar is 1 mm. (Reproduced with permission from A.R. Clarke and C.N. Eberhardt, Microscopy Techniques for Materials Science, Woodhead Publishing Ltd, Cambridge UK. 2002 Woodhead Publishing Ltd.)...

Fig. 15 Reprinted with permission from [103], copyright 2004 by the American Physical Society. Structure of a sheared suspension (diameter 1.42 pm) with strain amplitude /o = 0.38, frequency / = 30Hz and

EXPERIMENTAL FIGURE 11-29 Antibody and DNA probes colocalize SIRS protein with telomeric heterochromatin in yeast nuclei, (a) Confocal micrograph 0.3 jim thick through three diploid yeast cells, each containing 68 telomeres. Telomeres were labeled by hybridization to a fluorescent telomere-specific probe (yellow). DNA was stained red to reveal the nuclei. The 68 telomeres coalesce into a much smaller number of regions near the nuclear periphery, (b, c)... 

Confocal micrographs of yeast cells labeled with a telomere-specific hybridization probe (b) and a fluorescent-labeled antibody specific for SIRS (c). Note that SIRS is localized in the repressed telomeric heterochromatin. Similar experiments with RAR1, SIR2, and SIR4 have shown that these proteins also colocalize with the repressed telomeric heterochromatin. [From M. Gotta et al., 1996,... 

Figure 19. Confocal micrograph of an MFT sample that was unfrozen and observed using the confocal optical technique. The orientation or structuring of the clay component is identical to that observed with the cryogenic sample preparation for the electron microscope (Figure 18). Scale bar, 100 pms. Photograph courtesy of V. A. Munoz.

Figure 3.16 Comparison of the confocal micrographs of Stroock et al. [58] (left) with the Poincare sections obtained from Lagrangian particle tracking by Kang and Kwon [60] (right).

Figure 2. Colocalization of CD4 and SalplS on the surface of HeLa cells ectopically expressing CD4 (HeLa-CD4). Confocal micrograph corresponding to staining of surface CD4 with a specific antibody labeled with Alexa Fluor594 and SalplS labeled with Alexa Fluor4Bs.

Fig. 7 Three-dimensional twisting flow in a channel with obliquely oriented ridges on one wall, (a) Schematic of channel with ridges, (b) Optical micrograph showing a top view of a red stream and a green stream flowing on either side of a clear stream in the channel, (c) Fluorescent confocal micrographs of vertical cross sections of a microchannel such as in...

Figure 4. Confocal micrograph images ofTfMAA-coated QDs (a) alone (b) 5 min after addition of anti-TfmAb Panels (c) and (d) show images of a thioglycerol-coated QD alone and 45 min after addition of antibody, respectively.

Fig. 14 Confocal micrographs of radial artery cells seeded onto samples of PVA-C control, PVA-C soaked in fibronectin (FN), PVA-C-FN (FN-functiraialized PVA-C) prepared without CDI, and PVA-C-FN prepared with CDI. Cytoskeleton (red) was labeled with anti-smooth muscle a-actin-Cy3-conjugated Ig02a primary. Cell nuclei (blue) were labeled with Hoechst 33342. Scale bars 50 pm. For further experimental details, refta- to [110]. Reprinted from [110] with permission. Copyright (2011) Elsevier...

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