Competitive inhibition enzyme-linked immunosorbent

Muldoon et al. developed a monoclonal-based competitive inhibition enzyme-linked immunosorbent assay (cELISA) for sulfadimethoxine. The group compared... 

Soluble cytldylyltransferase protein levels were measured using a competitive Inhibition enzyme-linked Immunosorbent (ELISA) method . A polyclonal antibody was raised in rabbit to the purified cytldylyltransferase and this was used in the ELISA with a goat anti-rabbit IgG-horseradish peroxidase conjugate. The colorimetric reaction was obtained with a substrate containing hydrogen peroxide and 0-phenylenediamlne. ... 

Enzyme-Linked Immunosorbent Assays (ELISA). Three methods are commonly used direct competition, double antibody sandwish and antibody inhibition. 

The most common types of assays employed to quantitate protein concentrations in biological matrices are listed in Table 32.4. Enzyme-linked immunosorbent assays (ELISAs), radioimmunoassays (RIAs), and immunoradiometric assays (IRMAs) require protein-specific antibodies, labeled proteins, or labeled antibodies as reagents, and are generally competitive inhibition assays. Radioimmunoassays measure concentrations by displacing ligands from cell-bound receptors. The most common assay, the... 

Many types of assay are available to be used in HTS protocols to identify inhibitors of PPIs, but a competition assay, in which inhibition of complex formation is measured, is most common. Fluorescence polarization (FPA), fluorescence resonance energy transfer (FRET), enzyme-linked immunosorbent assays (ELISA), and other assay formats have been used. The interacting proteins can be used in their full-length forms though, more frequently only the interacting domains are employed, and if possible the excised interacting peptide is usually preferred. 

The procedures for production of specific antibodies and their application in a competitive inhibition ELISA (Enzyme-Linked Immunosorbent Assay) are discussed in detail in the preceding chapter (Vanderlaan et al., this volume). In addition, other comprehensive overviews of the immunoassay development process in the pesticide field are available in general, a... 

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