Column packing small bore columns

The separation was carried out on a small bore column 25 cm long and 1 mm i.d. packed with silica gel having a particle diameter of lOp. The mobile phase was n-hexane saturated with water and the flow rate 50 pi per min. 

Few laboratories have developed their own facilities for packing conventional and small bore columns, preferring to rely on commercial manufacturers for their needs. This is understandable since packed columns containing any common phase can be obtained at an acceptable cost and with a guarantee of acceptable performance and stability. Column packing generally requires access to equipment not readily available in all laboratories and... 

Slurry packing techniques are required for the preparation of efficient columns with rigid particles of less than 20 micrometers in diameter. The same general packing apparatus. Figure 4.8, can be used to pack columns by the balanced-density slurry, liquid slurry, or the viscous slurry techniques. Down-fill slurry packing is the method of choice for small bore columns and packed capillary columns. 

Figure 1.16 Separation ot a test mixture by adsorption chromatography on a 1 m x 1 mm I.D. small bore column packed with 8 aicrometer Zorbax B.P. Sil operated at a flow rate of ISO microliters/min (left) and a 22 m x 1 mm I.D. column of the same packing material prepared by series coupling of 1 m segments and operated at a flow rate of 15 microliters/min (right). (Reproduced with permission from ref. 234. Copyright American Chemical Society).

Knox, J. H., Theoretical aspects of LC with packed and open small-bore columns, /. Chromatogr. Sci., 18, 453, 1980. 

High performance liquid chromatography on a small bore column packed with microparticulate silica based ion exchange material has been used [317] to determine down to 0.01 mg L 1 nitrate in water without interference from other ions associated with potable, pond, river and stream water. 

Inlet systems for packed columns can often be used with capillary columns as well. However, the much smaller injection volumes and slower gas flow rates used with capillary columns, especially small-bore... 

Syringe pumps (section 5.2.2) are widely used with open tubular and packed capillary columns and occasionally with small bore columns having internal diameters less than 2 mm [3]. Typical syringe pumps have cylinder volumes of 100 or 250 ml and lack the... 

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