Column chromatography clinical laboratory

Automated, High-Resolution Analyses for the Clinical Laboratory by Liquid Column Chromatography... 

Faulkner (personal communication) in the same laboratory used column chromatography for clinical purposes and for fractionation of individual gastric juices. Nondialyzable solids from 50 ml gastric juice were applied to a 1 X 10 cm column and eluted with citrate and phosphate buflFers of increasing pH (3.2-8.0). Welsh et al. (W12, W12a)... 

AUTOMATED, HIGH-RESOLUTION ANALYSES FOR THE CLINICAL LABORATORY BY LIQUID COLUMN CHROMATOGRAPHY... 

Relatively few truly automated, high-resolution analytical systems are now used in the clinical laboratory. For this presentation, I have arbitrarily chosen only those systems that use column chromatography for separation. This choice is based not only on the ability of these systems to separate literally hundreds of the molecular constituents in a physiological fluid but also because they are directly amenable to a high degree of automation. Obviously, this latter point is extremely important for any future development in the clinical laboratory. Further, only liquid chromatography will be discussed here since there has recently... 

Yl. Young, D. S., High-pressure column chromatography of carbohydrates in the clinical laboratory. Amer. J. Clin. Pathol. 53, 803-810 (1970). 

Scott, C. D., Automated, high resolution analysis in the clinical laboratory by liquid column chromatography. Adv. Clin. Chem. 15, 1-41 (1972). 

Quantitative analysis of amino acids can be performed by an amino acid analyzer with ion-exchange column, a HPLC system with a reverse phase column, or gas chromatography. The amino acid analyzer has been the most popular method in clinical laboratories. Most of the normal and pathological values in the literature were obtained by this technique. Analysis by HPLC is now being used by an increasing number of laboratories. 

Ion-exchange chromatography has many clinical applications, including the separation of amino acids, peptides, proteins, nucleotides, oligonucleotides, and nucleic acids. Another important application of ion-exchange chromatography is the separation and removal of inorganic ions from aqueous mixtures. Thus most water purification units used to prepare deionized water for the laboratory contain "mixed-bed columns of cation and anion resins (see Chapter 1). 

Numerous laboratories have attempted to demonstrate a relation between the clinical efficiency of the intrinsic factor and its capacity to combine with the vitamin. If the purified intrinsic factor separates into several peaks, either by electrophoresis or by chromatography on a cellulose column, the fractions with the greatest capacity for binding the vitamin have the most active intrinsic capacities when measured clinically. 

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