Column chromatographic method

Mlcrochromatographlc Methods During the past two years rapid. Inexpensive, miniaturized column chromatographic methods for the separation of hemoglobins have been developed These methods are designed for the qualitative detection and quantitative determination of hemoglobins In normal and abnormal conditions and cover the quantitation of Hb-A2 the detection of Hb-S, Hb-C other abnormal Hbs differentiation of various conditions In adults and the detection of hemoglobinopathies especially sickle cell anemia at birth (27, 28, 29, 30) ... 

Gorcharova and Khomenko [127] have described a column chromatographic method for the determination of acetic, propionic, and butyric acids in seawater, and thin-layer chromatographic methods for determining lactic, aconitic, malonic, oxalic, tartaric, citric, and malic acids. The pH of the sample is adjusted to 8 - 9 with sodium hydroxide solution. It is then evaporated almost... 

Initially, progress in this area was hampered by the lack of suitable analytical methods for chiral hydrocarbons. Early studies relied on optical rotation to determine enantiomeric excess (ee) values, but with the development of chiral gas chromatography (GC) and high-performance liquid chromatography (HPLC) columns, chromatographic methods have become more common. 

A number of experiments are possible which will add final clarification to questions concerning the details of the mechanism. Modem column chromatographic methods can be used to evaluate the role of the adsorbent. In a column, no solvent concentration profile exists, and there are at least two papers which indicate adsorption separations can be successful (22, 23). More extensive work is necessary to characterize the solvent concentration profile to improve the quantitative agreement between bulk precipitation concentrations and in situ precipitations in TLC. 

Adequate column chromatographic methods are now opening the way toward the required progress, allowing for more complete and more accurate quantitative data on amino acid blood levels as well as on amino acid renal excretion. 

Some members of class A high-molecular-mass PBPs were found to bind to moenomycin [64,94], E. coli PBP la and lb were purified by using moenomycin-affinity column chromatography in combination with other column chromatographic methods. 

Open-column chromatographic methods are no longer widely used in quantifying drug poducts. Yet, a number of methods in the USP 23 [3] describe the use of open-column methods for sample pretreatment. Columns packed... 

From the known, differential complexing between boronic acids and polyhydroxy compounds, it follows that carbohydrate mixtures may be separated by column-chromatographic methods that exploit the differences. Nucleoside and nucleotide boronates have been separated on columns of anion-exchange resins,90 and sugars and alditols have been shown to be differentially retained on such resins in the sulfonated phenylboronic acid form,64 but perhaps the best uses of column chromatography in this connection have incorporated the resolving powers of insoluble polymers to which boronic acid groups have been covalently bonded. Such insoluble forms of boronates have been synthesized either by substitution of polysaccharide derivatives, or by polymerization of suitable arylboronic acids. 

Numerous of the column chromatographic methods described in this paper make possible or simultaneously included the enrichment of the separated elements (Chaps. 2.1 and 2.2). In this section only those publications are mentioned which concentrate on enrichment applications. 

Karajannis, S., Ortner, H. M., Spitzy, H. Column-chromatographic method for removal of the molybdenum matrix in determination of alkali and alkaline-earth metals in molybdenum and its compounds. Talanta 19, 903 (1972)... 

Compare supercritical-fluid chromatography with other column chromatographic methods. 

However, if molecules that are small enough to penetrate all the pores are present, then the whole of the mobile phase volume becomes available to them. As the mobile phase stagnates in the pores, diffusion is the only way in which molecules can escape and as a result they move more slowly than the excluded molecules. They are the last to appear in the detector, namely at the breakthrough time known from all the other column chromatographic methods. The solvent itself does not interact with the stationary phase but passes through all the pores, and solvent molecules are the first to appear in the detector in all other modes of... 

At this stage, one of two different column chromatographic methods may be used. The best method for final purification needs to be determined for the protein of interest (see Note 46). 

The author uses the classification of column chromatographic methods according the pressure applied ... 

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