Color combinatorial chemistry

Codon (mRNA), 1109-1110 table of, 1110 Coenzyme, 162, 1042 table of, 1044-1045 Coenzyme A, structure of, 817, 1044 Coenzyme Q, 632 Cofactor (enzyme), 1042 Color, perception of, 503-504 UV spectroscopy and. 503-505 Combinatorial chemistry, 586-587 kinds of, 586... 

Other approaches for presenting information to facilitate the visualization of meaningful patterns for rapid decision involve combinatorial chemistry-related applications. For example, methods for the analysis of combinatorial chemistry-derived samples provide visual representations of the 96-well plate (Figure 5.5) (Yates et al., 2001). Following the LC/MS analysis, an automated analysis is performed, according to preestablished thresholds to search for the protonated molecule ion of the analyte. If the ion is found, then the visual representation of the corresponding well is marked with a distinguishing color scheme. In this way, the scientist quickly inspects the visual representation to make decisions. 

Egner BJ, Rana S, Smith H, Bouloc N, Frey J, Brocklesby WS, Bradley M, Tagging in combinatorial chemistry the use of colored and fluorescent beads, Chem. Commun., pp. 735-736, 1997. 

FIGURE 13.5 (See color insert following page 114.) Partial view of screen in New Leads Generation (combinatorial chemistry and HTS) generating a huge quantity of hard-to-analyze results. 

G. Eckehard, R. Ramsey, and I. Lewis, High-throughput flow-injection analysis mass spectrometry with networked delivery of color-rendered results. 2. Three dimensional spectral mapping of 96-well combinatorial chemistry rack. Anal. Chem. 70, 3227-3234 (1998). 

The availability of several dye tags with several distinguishable excitation and emission has already been exploited for purposes of identifying combinatorial chemistry libraries on beads, but the broad nature of molecular absorption and emission spectra puts a bandwidth limit on the number of dyes that can be practically applied. The logic approach mentioned in the previous paragraph can increase the diversity available with a single color combination manifold times. 

In this chapter several examples were used in the validation of chemistry and precursors. They illustrate the importance of FTIR and color tests in aiding the development of solid-phase chemistry in the loading, diversification, and cleaving steps in combinatorial synthesis. The combined method provides crucial information on reactions carried out on solid supports and offers a fast way to monitor a range of reactions without the need to cleave the compound from resin at intermediate steps. Building block reactivity for similar reactions can also be achieved to guide future synthesis development, as these data may prove powerful for efficient and cost-effective combinatorial library development. 

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