Collagen electron micrographs

Fig.1. Electron micrograph of a mast cell in human heart tissue. The cytoplasm contains numerous secretory granules. The mast cell is adjacent to a coronary blood vessel, surrounded by collagen fibers and close to a myocyte. Uranyl acetate and lead citrate stained. Orig. magnif. lO.OOOx. 

Forbes MJ (1980) Cross-flow filtration, Transmission electron micrographic analysis and blood compatibility testing of collagen composite materials for use as vascular prostheses. M.S. Thesis, Massachusetts Institute of Technology, Cambridge, MA... 

Electron micrograph of a polygonal network formed by association of collagen type IV monomers. 

Figure 8-1 Electron micrograph of a thin section of a fat storage cell or adipocyte. L, the single large fat droplet N, nucleus M, mitochondria En, endothelium of a capillary containing an erythrocyte (E) CT, connective tissue ground substance which contains collagen fibers (Co) and fibroblasts (F). The basement membranes (BM) surrounding the endothelium and the fat cell are also marked. From Porter and Bonneville.6 Courtesy of Mary Bonneville.

Figure 29-10 Schematic diagram of collagen molecules in a fibril so arranged as to give the 640-A spacing visible in electron micrographs...

An electron micrograph of collagen fibrils from skin. (Courtesy of Jerome Gross, Massachusetts Genera] Hospital.)... 

Ronziere, M-C., Herbage, B., Herbage, D., and Bemengo, J-C. (1998). Fourier analysis of electron micrographs of positively stained collagen fibrils Application to type I and II collagen typing. Int. J. Biol. Macromol. 23, 207-213. 

Fig. 1. (A) Scanning electron micrograph of human skin. The epidermis has pulled away from part of the basement membrane. (B and C) Transmission electron micrograph through the epidermal-dermal junction of human skin. Keratinocytes (KF) are the cells in the human epidermis. LD, The lamina densa of the basement membrane LL, the lamina lucida. Typical anchoring fibrils (AF) formed from type VII collagen are shown at higher power in C. Courtesy of Dr. K. Holbrook, University of Washington.

Fig. 3. Electron micrographs of type IV collagen (a), laminin (b), and nidogen (c) molecules after rotary shadowing. Bar in a-c, 50 nm.

Figure 3.29. Relationship between proteoglycans and collagen fibrils in tendon. Transmission electron micrograph showing positive staining pattern of type I collagen fibrils from rabbit Achilles tendon stained with quinolinic blue. Proteoglycan filaments (arrows) are shown attached to collagen fibrils.

Figure 5.12. Lateral fusion of collagen fibrils during fascicle development of chick extensor tendon. Transmission electron micrograph showing the lateral fusion of collagen fibrils at day 17 of chick embryogenesis. Note that the demarcation between collagen fibrils (arrows) is less clear compared to the cross section shown at day 14 (Figure 5.11). Several fibrils appear to be in the process of fusion generating fibrils with irregular cross sections. The fibril bundle (fiber) diameter is still about 2 pm before fusion similar to that observed on day 14 (see Silver et al., 2003).

Figure 1.6. Collage of transmission electron micrographs of embedded and stained sections through rat predentin tissue, showing all the maturation stages from the cells to the mineralized material. The banded collagen fibers appear increasingly tightly packed through the predentin layers up to the mineralization front (MF), where they...

All of the invertebrate collagens which have been examined have yielded the typical collagen wide-angle X-ray diffraction pattern, and electron micrographs of the fibers show, with the exception of Physalia float and Metridium body wall, the characteristic 600-700 A axial period (Gross et al., 1958). 

Fig. 12. Electron micrograph of air-dried, chromium-shadowed collagen fibrils from adult human corium magnification X 26,000, (From Gross and Schmitt, 1948.)...

---