Collagen bovine nasal-cartilage

Chondroitin sulfate could be separated from the chondromucoid. It remained in solution at pH 7 when chondromucoid was shaken with chloroform - amyl alcohol, whereas a second carbohydrate component (possibly keratosulfate or a closely related substance) remained with the protein fraction (4). Electrophoretic studies of chondromucoid show that the components associate in one kind of complex over the pH range 5 to 9 and that a different type of complex occurs below pH 4.85 (71). Shatton and Schubert (7 ) found both chondromucoid and free chondroitin sulfate in the extracts of bovine nasal cartilage the mucoid was extracted first. Protein denaturants did not separate the protein portion of the mucoid. The protein was not collagen (72). Since hyaluronic acid does not appear to... 

The chondroitin sulfate of animal cartilage is largely bound up as a complex with protein which is not derived from collagen (M2). The complex from bovine nasal septa contains 30% protein, 60% chondroitin... 

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