Coiled coil design

Woolfson s review provides a summary of the state of the art in coiled-coil design, and provides a keen insight into both the future potential of design methods and the challenges that they face. 

II. Rules for Coiled-Coil Design The Basics of Coiled-Coil... 

The review is laid out as follows in Part II, there is a discussion of the structural features of the coiled-coil assemblies and the current state-of-the-art of sequence-to-structure rules pertinent to design in Part III, there is a detailed description of some of the key coiled-coil designs, including the rules used to create them and their significance in the field and, finally, there is a discussion of the potential for new coiled-coil designs and where the field might go in the future. 

On this basis, coiled-coil designers often place oppositely charged residues Lys and Glu at complementary g and e sites to direct the assemblies that they are targeting (positive design), and make similar potential interactions in alternative structures repulsive (negative design). 

Coiled Coils Designed to Switch Conformational State (2002 and 2003)... 

Hodges, R. S., Saund, A. K., Chong, P. C. S., Stpierre, S. A., and Reid, R. E. (1981). Synthetic model for 2-stranded alpha-helical coiled-coils - design, synthesis, and characterization of an 86-residue analog of tropomyosin. / Biol. Chem. 256, 1214-1224. 

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